Description

Hirudin (54-65) (Desulfated) is a specific, chemically modified fragment of the potent natural thrombin inhibitor hirudin. This desulfated variant is a critical research tool for studying the structure-activity relationships of hirudin and the mechanisms of thrombin inhibition without the influence of the sulfate group. It is primarily utilized by pharmaceutical researchers, biochemists, and academic institutions engaged in cardiovascular research, anticoagulant drug development, and proteomics studies to elucidate specific binding interactions.

Application

• Biochemical Research: A fundamental reagent for investigating the precise binding mechanism between hirudin fragments and the thrombin enzyme.
• Structure-Activity Relationship (SAR) Studies: Used to compare and contrast inhibitory activity with native sulfated hirudin fragments to understand the role of tyrosine sulfation.
• Anticoagulant Mechanism Profiling: Serves as a control or specific probe in assays designed to study alternative pathways of thrombin inhibition.
• Pharmaceutical Development: Employed in early-stage R&D for novel antithrombotic agents, helping to design hirudin-based therapeutics.
• Protease Inhibition Studies: Useful in enzymatic studies to understand the specificity and kinetics of thrombin and related serine proteases.
• Academic & Clinical Research: Supports fundamental and applied research in hematology, thrombosis, and hemostasis.

Basic Information

Product Name	Hirudin (54-65) (Desulfated)
CAS No.	113274-56-9
Molecular Formula	C58H89N19O21
Molecular Weight	1380.45 g/mol
Synonyms	Hirudin Fragment 54-65 (Desulfated); Desulfato-Hirudin (54-65); [Desulfato-Tyr63]-Hirudin (54-65); Hirudin (54-65), Desulfated; Thrombin Inhibitor Peptide (Desulfated); HIR(54-65) (Desulfated); Leu-Val-Pro-Gln-Ser-Tyr(Desulfated)-Asn-Gly-Lys-Pro
EINECS	Contact for details

Quality Control

Our Hirudin (54-65) (Desulfated) is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and peptide content. We adhere to rigorous analytical standards, utilizing techniques such as HPLC and mass spectrometry to verify product integrity. Certificates of Analysis are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption. For short-term use, store at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.