Description

For-Met-Leu-Pna is a specialized peptide derivative, specifically a tripeptide substrate, widely utilized in biochemical and pharmaceutical research. This compound is essential for studying enzyme kinetics and inhibition, particularly for proteases like thrombin and other serine proteases. It serves as a critical tool for researchers and developers in the fields of pharmaceutical development, diagnostic assay design, and proteomics. Its precise structure makes it valuable for high-throughput screening and mechanistic studies.

Application

• Protease Substrate: Primary use as a chromogenic or fluorogenic substrate for serine proteases, including thrombin, trypsin, and related enzymes in kinetic assays.
• Pharmaceutical Research: Employed in drug discovery programs to screen for and characterize novel protease inhibitors for therapeutic applications.
• Diagnostic Development: Used in the development of in vitro diagnostic kits for measuring protease activity in clinical and research samples.
• Biochemical Tool: A key reagent for studying enzyme specificity, catalytic mechanisms, and protein-protein interactions in academic and industrial laboratories.
• Quality Control: Acts as a reference standard in the quality control of enzyme preparations and related biopharmaceutical products.

Basic Information

Product Name	For-Met-Leu-Pna
CAS No.	111150-07-3
Molecular Formula	C24H35N5O6S
Molecular Weight	521.63 g/mol
Synonyms	Formyl-Methionyl-Leucyl-P-Nitroanilide; Formyl-Met-Leu-pNA; F-M-L-pNA; N-Formyl-Met-Leu-pNA; Thrombin Substrate I; Boc-Met-Leu-pNA (common mis-assignment); Protease Substrate For-Met-Leu-pNA; N-Formyl-L-methionyl-L-leucyl-L-phenylalanine 4-nitroanilide
EINECS	Contact for details

Quality Control

Our For-Met-Leu-Pna is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analysis including HPLC for purity, identity confirmation by mass spectrometry (MS) and infrared spectroscopy (IR), and verification of correct optical rotation. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate and keep under inert atmosphere if possible. Allow the sealed container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.