Description

N,N,O,o-Tetrakis(tert-Butyldimethylsilyl)Methotrexate is a highly functionalized, silyl-protected derivative of the antifolate drug methotrexate. This advanced chemical intermediate is critical for researchers and manufacturers requiring precise control over methotrexate's reactive sites during complex synthetic sequences. It is primarily utilized by pharmaceutical R&D teams and fine chemical producers engaged in the development of novel drug conjugates, prodrugs, and targeted therapeutic agents.

Application

• Pharmaceutical Intermediate: A key protected intermediate in the synthesis of novel methotrexate-based drug candidates, prodrugs, and antibody-drug conjugates (ADCs).
• Medicinal Chemistry Research: Enables selective modification and derivatization of the methotrexate molecule for structure-activity relationship (SAR) studies.
• Bioconjugation Chemistry: The tert-butyldimethylsilyl (TBDMS) protecting groups allow for controlled, stepwise reactions in the preparation of targeted therapeutic constructs.
• Process Chemistry Development: Used in scaling up synthetic routes for methotrexate analogs under controlled, moisture-sensitive conditions.
• Academic & Institutional Research: Serves as a specialized reagent in laboratories studying folate metabolism, enzyme inhibition, and cancer therapeutics.

Basic Information

Product Name	N,N,O,o-Tetrakis(tert-Butyldimethylsilyl)Methotrexate
CAS No.	109736-06-3
Molecular Formula	C53H94N8O6Si4
Molecular Weight	1043.8 g/mol
Synonyms	MTX-TBDMS; Methotrexate TBDMS Derivative; N,N,O,o-Tetrakis[(1,1-dimethylethyl)dimethylsilyl]methotrexate; 4-[[2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic Acid Tetrakis(TBDMS) Ester; Fully Silylated Methotrexate; TBDMS-Methotrexate; Protected Methotrexate Intermediate
EINECS	Contact for details

Quality Control

Our N,N,O,o-Tetrakis(tert-Butyldimethylsilyl)Methotrexate is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity for research and development applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles. We adhere to cGMP principles where applicable to support our clients' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the product label or COA. This compound is hygroscopic (moisture-sensitive) and should be handled under an inert atmosphere (e.g., nitrogen or argon) when opening to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.