Description

Val-Ala p-Nitroanilide Acetate Salt is a specialized chromogenic peptide substrate used extensively in biochemical research and diagnostic applications. This compound serves as a critical reagent for the detection and quantification of specific protease enzyme activity, offering high sensitivity and selectivity. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, clinical diagnostics, and life science research to study enzyme kinetics and develop assay systems.

Application

• Protease Enzyme Assays: As a specific chromogenic substrate for serine proteases and other enzymes that cleave at the Val-Ala bond.
• Pharmaceutical Research: High-throughput screening (HTS) for identifying and characterizing protease inhibitors as potential drug candidates.
• Diagnostic Kit Development: A key component in the formulation of diagnostic tests for conditions involving abnormal protease activity.
• Biochemical Research: Used in enzymology studies to determine kinetic parameters (Km, Vmax) and elucidate enzyme mechanisms.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of therapeutic proteins and antibodies.
• Cell Biology Studies: Investigating the role of specific proteases in cellular processes like apoptosis and signal transduction.

Basic Information

Product Name	Val-Ala p-Nitroanilide Acetate Salt
CAS No.	108321-94-4
Molecular Formula	C16H23N5O5 • C2H4O2
Molecular Weight	439.44 g/mol
Synonyms	Val-Ala-pNA Acetate Salt; N-Valyl-L-alanine 4-nitroanilide acetate; L-Valyl-L-alanine 4-nitroanilide acetate; V-A-pNA Acetate; Val-Ala-NA Acetate; Protease Substrate Val-Ala-pNA; N-Succinyl-L-valyl-L-alanyl-L-lysine 4-nitroanilide acetate (related); Substrate for Granzyme B and Cathepsin C
EINECS	Contact for details

Quality Control

Our Val-Ala p-Nitroanilide Acetate Salt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, essential for reproducible research and assay performance. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzyme assays. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines
Biological Activity	Suitable for use in specified enzyme assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.