Description

H-D-Ala-Leu-Lys-Amc CAS NO 104881-72-3 is a specialized fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like trypsin and plasmin. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for high-throughput screening, enzyme kinetics studies, and biochemical research.

Application

• Protease Activity Assays: As a fluorogenic substrate for the quantitative determination of trypsin, plasmin, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors or activators.
• Biochemical Research: A key tool for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial R&D settings.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for conditions involving abnormal protease levels.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of therapeutic proteins and antibodies.

Basic Information

Product Name	H-D-Ala-Leu-Lys-Amc
CAS No.	104881-72-3
Molecular Formula	C₂₃H₃₆N₆O₅
Molecular Weight	476.57 g/mol
Synonyms	D-Ala-Leu-Lys-AMC; H-D-Ala-Leu-Lys-7-amido-4-methylcoumarin; Ala-Leu-Lys-AMC; Boc-D-Ala-Leu-Lys-AMC (precursor form); Fluorogenic substrate for trypsin; D-Ala-Leu-Lys-MCA; AMC-labeled tripeptide substrate; 7-Amido-4-methylcoumarin conjugate of H-D-Ala-Leu-Lys
EINECS	Contact for details

Quality Control

Our H-D-Ala-Leu-Lys-Amc is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant guidelines for research-grade biochemicals to support your regulatory and reproducibility requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.