Description

n-T-Boc-Ile-Glu-Gly-Arg 7-Amido-4-Methyl-Coumarin Hydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity protease activity assays. This compound matters for its role as a critical tool in biochemical research and pharmaceutical development, enabling precise measurement of enzyme kinetics and inhibitor screening. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors for studying proteolytic enzymes like thrombin and trypsin.

Application

• Protease Activity Assays: A key fluorogenic substrate for the continuous, real-time kinetic measurement of serine protease activity, including thrombin, trypsin, and related enzymes.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: Essential for fundamental research into enzyme mechanisms, specificity, and regulation within various biological pathways.
• Diagnostic Development: Serves as a core component in developing in-vitro diagnostic tests for conditions related to coagulation and other proteolytic processes.
• Quality Control in Biologics: Employed in the QC testing of biopharmaceuticals to monitor and validate the activity and purity of enzyme-based products.

Basic Information

Product Name	n-T-Boc-Ile-Glu-Gly-Arg 7-Amido-4-Methyl-Coumarin Hydrochloride
CAS No.	102601-26-3
Molecular Formula	C37H57N9O11 • HCl
Molecular Weight	840.37 g/mol (Free base: 803.91 g/mol)
Synonyms	Boc-Ile-Glu-Gly-Arg-AMC • HCl; t-Boc-Ile-Glu-Gly-Arg-AMC hydrochloride; Boc-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride; Fluorogenic Thrombin Substrate I; AMC-labeled Boc-IEGR peptide; Protease Substrate for Factor Xa and Thrombin; IEGR-AMC, Boc-; (7-Amino-4-methylcoumarin-4-yl)acetyl-Ile-Glu-Gly-Arg, N-t-Boc-
EINECS	Contact for details

Quality Control

Our n-T-Boc-Ile-Glu-Gly-Arg 7-Amido-4-Methyl-Coumarin Hydrochloride is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity verification. Certificates of Analysis (COA) detailing specific results for purity, absorbance, and functional activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorbance (UV-Vis)	Conforms to reference spectrum
Water Content (KF)	≤ 5.0%
Functional Activity	≥ 90% of reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.