Description

Rec Acetyl-Eglin C CAS NO 102188-32-9 is a modified, recombinant form of the potent elastase inhibitor Eglin c, featuring an acetylated N-terminus. This engineered peptide is crucial for advanced biochemical research and development, offering enhanced stability and specific inhibitory activity. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and biotechnology companies focused on protease inhibition studies, inflammatory pathway analysis, and novel therapeutic agent development.

Application

• Protease Inhibition Research: Serves as a specific and potent inhibitor for studying serine proteases like human leukocyte elastase (HLE), cathepsin G, and proteinase 3 in in vitro assays.
• Inflammatory Disease Models: Used in preclinical research to investigate the role of neutrophil elastase in conditions such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and acute respiratory distress syndrome (ARDS).
• Biopharmaceutical Development: Acts as a reference standard or a lead compound in the development of anti-inflammatory and anti-protease therapeutic agents.
• Structural Biology Studies: Employed in X-ray crystallography and NMR spectroscopy to elucidate protease-inhibitor complex structures and mechanisms of action.
• Enzyme Kinetics: A key tool for determining kinetic parameters (Ki, IC50) and studying the thermodynamics of enzyme-inhibitor interactions.
• Cell Culture & Tissue Engineering: Applied in cell-based assays to modulate protease activity and study its impact on cellular signaling, extracellular matrix remodeling, and inflammatory responses.

Basic Information

Product Name	Rec Acetyl-Eglin C
CAS No.	102188-32-9
Molecular Formula	C173H274N50O55S2
Molecular Weight	~3950.4 g/mol (monoisotopic)
Synonyms	Acetylated Recombinant Eglin c; N-Acetyl-Eglin c; rAc-Eglin C; Eglin C, N-acetyl-; Recombinant Eglin C (acetylated); Protease Inhibitor Eglin c (acetylated); Hirudinaria manillensis protease inhibitor homolog (acetylated); LEI (acetylated).
EINECS	Contact for details

Quality Control

Our Rec Acetyl-Eglin C is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Quality is verified through a comprehensive suite of analytical techniques including RP-HPLC for purity, mass spectrometry for identity confirmation, and amino acid analysis. Each batch is supplied with a detailed Certificate of Analysis (COA) documenting purity, peptide content, endotoxin levels, and biological activity (inhibitory potency). We adhere to GMP principles for manufacturing to support research and pre-clinical development needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Mass Spec)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Biological Activity (IC50 vs. HLE)	≤ 0.5 nM
Endotoxin Level	< 1.0 EU/mg
Solubility	Soluble in water and aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.