Description

L-Isoleucine-Ul-14C is a uniformly carbon-14 labeled isotopologue of the essential branched-chain amino acid L-Isoleucine, identified by CAS NO 18875-42-8. This radiolabeled compound is a critical tool for metabolic pathway tracing, enabling precise quantification and visualization of amino acid uptake, distribution, and catabolism in biological systems. It is indispensable for researchers and quality control laboratories in the pharmaceutical, nutritional science, and biochemical research sectors who require high-specific-activity tracers for advanced in vitro and in vivo studies.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used as a radiolabeled tracer to investigate the absorption, distribution, metabolism, and excretion (ADME) profiles of new chemical entities and biologics.
• Nutritional and Metabolic Research: Essential for studying protein synthesis, turnover, and the specific metabolic fate of L-Isoleucine in muscle tissue and other organs.
• Biochemical Pathway Analysis: Enables detailed mapping of branched-chain amino acid (BCAA) catabolic pathways and their regulation in health and disease states.
• In Vitro Diagnostic Assay Development: Serves as a key component in the development and validation of sensitive radioimmunoassays (RIA) and other ligand-binding assays.
• Agricultural and Animal Science Research: Applied in studies of feed efficiency, amino acid utilization, and metabolic studies in livestock.
• Quality Control Reference Standard: Acts as an internal standard in mass spectrometry and other analytical techniques for quantifying unlabeled L-Isoleucine in complex matrices.

Basic Information

Product Name	L-Isoleucine-Ul-14C
CAS No.	18875-42-8
Molecular Formula	C14C5H13NO2 *or* C6H13NO2 (¹⁴C labeled)
Molecular Weight	~131.17 g/mol (unlabeled base)
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic acid-Ul-14C; (2S,3S)-2-Azaniumyl-3-methylpentanoate-Ul-14C; L-2-Amino-3-methylvaleric acid-Ul-14C; (S)-Isoleucine-Ul-14C; L-α-Amino-β-methylvaleric acid-Ul-14C; Ile-Ul-14C; I-Ul-14C; Uniformly Carbon-14 Labeled L-Isoleucine
EINECS	Contact for details

Quality Control

Every batch of L-Isoleucine-Ul-14C is manufactured and analyzed under strict quality systems to ensure the highest standards of radiochemical purity, chemical purity, and specific activity. Our quality commitment includes comprehensive testing via HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting radionuclidic purity, radiochemical purity, specific activity, and chemical identity. Production follows cGMP guidelines where applicable to support regulatory filings.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under inert atmosphere or in a controlled, dry environment to maintain stability and specific activity. For long-term storage, keep containers sealed in secondary packaging.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC, MS)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Specific Activity	50-60 mCi/mmol (Custom ranges available)
Solvent (for solutions)	Specified on COA (e.g., Ethanol:Water)
Radioactivity Concentration	As specified on COA (e.g., 1.0 mCi/mL)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.