Description

5'-O-(4,4'-Dimethoxytrityl)-Guanosine is a key nucleoside derivative, specifically a guanosine molecule protected at the 5'-hydroxyl position with a dimethoxytrityl (DMT) group. This protection is critical for controlling regioselectivity during automated oligonucleotide synthesis, ensuring high coupling efficiency and purity in the final nucleic acid sequence. It is an essential phosphoramidite building block for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors who require precise and reliable synthesis of DNA and RNA oligonucleotides for applications like PCR primers, probes, and gene synthesis.

Application

• Solid-Phase Oligonucleotide Synthesis: As a protected monomer (typically converted to the corresponding phosphoramidite) for the automated, stepwise construction of DNA and RNA strands.
• Antisense Oligonucleotide & siRNA Production: A fundamental raw material in the manufacture of therapeutic nucleic acids designed to modulate gene expression.
• PCR Primer and Probe Manufacturing: Used to synthesize specific, high-purity oligonucleotide sequences essential for molecular diagnostics and genetic research.
• Gene Synthesis and Cloning: Serves as a building block for the de novo synthesis of long DNA fragments and genes.
• Research & Development: A vital reagent in academic and industrial labs for studying nucleic acid chemistry, developing novel assays, and creating synthetic biology tools.
• Diagnostic Kit Component Production: Integrated into the supply chain for producing key components of diagnostic tests, including those for infectious diseases and genetic disorders.

Basic Information

Item	Detail
Product Name	5'-O-(4,4'-Dimethoxytrityl)-Guanosine
CAS No.	17331-23-6
Molecular Formula	C₃₁H₃₁N₅O₇
Molecular Weight	585.61 g/mol
Synonyms	5'-O-DMT-Guanosine; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine (common protected form); Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; DMT-Guanosine; 5'-O-(Bis(4-methoxyphenyl)phenylmethyl)guanosine; 1-[(2R,3R,4S,5R)-3,4-Dihydroxy-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]oxolan-2-yl]-5-hydroxy-2-imino-3H-purin-6-one; DMTr-G
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-Guanosine is produced and tested under strict quality management systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to guarantee consistency and performance. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive) and should be kept under anhydrous conditions, ideally in a desiccator, to maintain stability and prevent decomposition. For long-term storage, consider storing at -20°C under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.