Description

Acetamide, N-[1-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethysilyl]-β-L-ribofuranosyl]-1,2-dihydro-2-oxo-4-pyrimidinyl]- is a sophisticated nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise, high-yield incorporation of modified nucleosides into DNA and RNA sequences, which is essential for advanced research and therapeutic development. This high-purity intermediate is indispensable for pharmaceutical R&D, biotechnology companies, and academic laboratories focused on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer for the automated synthesis of modified DNA/RNA strands.
• Therapeutic Oligonucleotide Development: Used in the research and production of antisense drugs, siRNA molecules, and aptamers for targeted therapies.
• Diagnostic Probe Manufacture: Enables the creation of labeled and modified nucleic acid probes for diagnostic assays and genomic research.
• Structural Biology Studies: Facilitates the synthesis of oligonucleotides with specific modifications to study nucleic acid structure, function, and protein interactions.
• Next-Generation Sequencing (NGS): Used in the preparation of specialized oligonucleotide libraries and primers.
• CRISPR/Cas9 System Components: Applied in the synthesis of guide RNAs (gRNAs) with enhanced stability or functionality.

Basic Information

Product Name	Acetamide, N-[1-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-L-ribofuranosyl]-1,2-dihydro-2-oxo-4-pyrimidinyl]-
CAS No.	237060-94-5
Molecular Formula	C55H71N5O10PSi
Molecular Weight	1029.24 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-N4-Acetyl-cytidine-3'-CE Phosphoramidite (β-L isomer); L-Cytidine, 5'-O-(4,4'-dimethoxytrityl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-N4-acetyl-, 3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; β-L-Cytidine CE Phosphoramidite (Ac, 5'-DMT, 2'-TBDMS); N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-β-L-cytidine-3'-O-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; L-Cytidine phosphoramidite monomer; Modified Cytidine Phosphoramidite; Contact for additional trade names.
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control protocols in a GMP-compliant environment. Every batch is characterized using advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to ensure identity, purity, and reactivity for sensitive oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with each shipment to guarantee batch-to-batch consistency and traceability for your regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive and light-sensitive nature. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.