Description

(T-4)-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-5-[[5-(4-methoxyphenyl)-2H-pyrrol-2-ylidene-κN]methyl]-2,4-dimethyl-1H-pyrrole-3-propanamidato-κN1]difluoroboron is a sophisticated organoboron complex featuring a BODIPY (boron-dipyrromethene) core functionalized with an N-hydroxysuccinimide (NHS) ester linker. This compound matters as a highly reactive and versatile labeling reagent, designed for the covalent conjugation of biomolecules such as proteins, peptides, and antibodies. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for creating fluorescent probes and assays.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to primary amines (-NH2) on proteins, antibodies, and peptides for fluorescence microscopy, flow cytometry, and immunoassays.
• Diagnostic Probe Development: Synthesis of targeted fluorescent probes for in vitro and in vivo imaging applications.
• Bioconjugation & Chemical Biology: A key building block for creating bioconjugates to study protein interactions, localization, and function.
• Sensor Fabrication: Integration into polymeric or solid-phase materials to develop fluorescence-based chemical or biological sensors.
• Pharmaceutical Research: Used in drug discovery for labeling candidate molecules to track their distribution and metabolism.
• Material Science: Functionalization of nanoparticles or surfaces with fluorescent tags for tracking and analysis.

Basic Information

Product Name	(T-4)-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-5-[[5-(4-methoxyphenyl)-2H-pyrrol-2-ylidene-κN]methyl]-2,4-dimethyl-1H-pyrrole-3-propanamidato-κN1]difluoroboron
CAS No.	217190-15-3
Molecular Formula	C₃₃H₃₈BF₂N₄O₆
Molecular Weight	635.49 g/mol
Synonyms	BODIPY FL NHS Ester; BODIPY® FL NHS Ester; BODIPY-FL, SE; 4,4-Difluoro-5,7-Dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid, N-succinimidyl ester; BDP FL NHS ester; BODIPY 493/503 NHS Ester; 4,4-Difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-8-propionic acid, succinimidyl ester (common naming variant)
EINECS	Contact for details

Quality Control

Our BODIPY FL NHS Ester is produced under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification and spectroscopic methods for identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key specifications. We adhere to cGMP principles where applicable to support our customers in regulated research and development environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	~503 nm
Emission (λmax)	~512 nm
Extinction Coefficient (ε)	~80,000 cm⁻¹M⁻¹
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.