Description

Chaps CAS NO 75621-03-3 is a zwitterionic detergent widely recognized for its ability to solubilize membrane proteins while maintaining their native structure and function. This compound is essential for researchers and manufacturers requiring gentle yet effective protein extraction and stabilization. It is a critical reagent for biotechnology, pharmaceutical development, and life science research applications, particularly in the study of membrane-bound proteins and enzymes.

Application

• Membrane Protein Solubilization: Gently extracts integral membrane proteins from lipid bilayers for downstream analysis.
• Protein Stabilization: Maintains the activity and conformation of sensitive enzymes and receptors in solution.
• Chromatography: Used in the purification of membrane proteins via techniques like affinity and size-exclusion chromatography.
• Cell Lysis: An effective component in lysis buffers for isolating intracellular proteins without denaturation.
• Diagnostic Assays: Employed in buffer systems for immunoassays and other diagnostic tests to ensure protein integrity.
• Structural Biology: A key detergent for preparing samples for X-ray crystallography and cryo-electron microscopy.
• Biopharmaceutical Manufacturing: Used in process development for the production of therapeutic proteins and vaccines.

Basic Information

Product Name	Chaps
CAS No.	75621-03-3
Molecular Formula	C32H58N2O7S
Molecular Weight	614.88 g/mol
Synonyms	3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate; CHAPS; 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate; N,N-Dimethyl-3-sulfo-N-[3-[(3α,5β,7α,12α)-3,7,12-trihydroxy-24-oxocholan-24-yl]aminopropyl]propan-1-aminium; Zwittergent 3-08
EINECS	Contact for details

Quality Control

Our Chaps is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for critical research and production applications. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a desiccated environment to prevent absorption of atmospheric moisture, which can affect product performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
pH (10% solution)	5.0 - 7.0
Critical Micelle Concentration (CMC)	6 - 10 mM

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.