Description

2,3,5-Triphenyltetrazolium Bromide is a vital organic salt widely used as a redox indicator and a biochemical reagent. Its core value lies in its ability to form a vivid, water-insoluble red formazan dye upon reduction, making it an essential tool for detecting metabolic activity and cellular viability. This compound is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors for applications ranging from microbiology to histochemistry.

Application

• Microbiological Assays: Used as a vital stain to differentiate between viable and non-viable microorganisms, particularly in sterility testing and antimicrobial susceptibility assays.
• Cell Viability & Cytotoxicity Testing: Serves as a key substrate in MTT and related assays to measure mitochondrial activity and cell proliferation in mammalian cell cultures.
• Seed Viability Testing: Applied in agricultural research to assess seed germination potential and vigor by staining metabolically active embryo tissues.
• Histochemistry & Staining: Employed as a marker for dehydrogenase enzyme activity in tissue sections, aiding in the localization of metabolic processes.
• Biochemical Research: Functions as an electron acceptor in numerous enzymatic assays, particularly for dehydrogenases, in research and diagnostic kits.
• Pharmaceutical Quality Control: Utilized in potency tests and sterility checks for injectable products and other sterile pharmaceuticals.

Basic Information

Product Name	2,3,5-Triphenyltetrazolium Bromide
CAS No.	1096-80-6
Molecular Formula	C19H15BrN4
Molecular Weight	379.25 g/mol
Synonyms	TPTZ Bromide; TTC Bromide; Triphenyltetrazolium Bromide; Tetrazolium Red; 2,3,5-Triphenyl-2H-tetrazolium Bromide; TPB; Red Tetrazolium; NSC 1770
EINECS	214-142-5

Quality Control

Our 2,3,5-Triphenyltetrazolium Bromide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including identification by IR spectroscopy, purity assay by HPLC, and control of specific impurities, to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept tightly sealed after each use to prevent degradation from moisture and light exposure.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility Test	Clear, colorless to pale yellow solution in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.