Description

4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Hemisodium Salt is a high-purity zwitterionic organic compound widely used as a biological buffer. This specialized hemisodium salt form offers enhanced solubility and stability for critical applications requiring precise pH control. It is an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries, where maintaining physiological pH conditions is paramount.

Application

• Biochemical & Molecular Biology Research: As a key component in buffer systems for cell culture media, enzyme assays, protein purification, and electrophoresis.
• Pharmaceutical Formulation: Used in the development and manufacturing of biologics, vaccines, and other parenteral drugs to stabilize pH.
• Diagnostic Reagents: Incorporated into buffers for clinical diagnostic kits and assays to ensure accuracy and reproducibility.
• Tissue Culture & Cell-Based Assays: Provides a stable, non-toxic environment for mammalian cell growth and viability studies.
• Chromatography: Serves as a mobile phase additive or equilibration buffer in various chromatographic techniques, including HPLC.

Basic Information

Product Name	4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Hemisodium Salt
CAS No.	103404-87-1
Molecular Formula	C8H18N2O4S • 0.5Na
Molecular Weight	Approx. 260.3 g/mol (monoisotopic)
Synonyms	HEPES Hemisodium Salt; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic Acid Hemisodium Salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) Hemisodium Salt; HEPES Sodium Salt (hemi); 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic Acid Hemisodium Salt; HEPES Na (0.5); PIPES Derivative Hemisodium Salt
EINECS	Contact for details

Quality Control

Our 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Hemisodium Salt is manufactured under strict quality management systems. Each batch is subjected to rigorous analytical testing, including HPLC for purity, to ensure it meets the high standards required for research and production. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after use to prevent moisture absorption, which can affect stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.5%
pH (1% Solution)	6.5 - 8.0
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 10 ppm
Endotoxin	< 0.1 EU/mg (for applicable grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.