Description

Ethidium Bromide is a vital fluorescent nucleic acid stain widely used in molecular biology for visualizing DNA and RNA in agarose and polyacrylamide gels. Its high binding affinity and fluorescence enhancement upon intercalation make it an indispensable tool for research and diagnostic applications. This product is essential for laboratories in biotechnology, pharmaceutical development, academic research, and clinical diagnostics requiring reliable and sensitive detection of nucleic acids.

Application

• Gel Electrophoresis: Primary stain for visualizing DNA and RNA bands in agarose and polyacrylamide gel electrophoresis.
• Nucleic Acid Quantification: Used in fluorometric assays to determine the concentration of DNA and RNA samples.
• Cytogenetics: Employed as a chromosome and nuclear stain in fluorescence microscopy.
• Viability Assays: Utilized to distinguish between live and dead cells in microbiology and cell biology.
• Mutation Detection: Applied in techniques for identifying nucleic acid conformational changes and mutations.
• Research & Development: A fundamental reagent in molecular cloning, PCR product analysis, and genetic fingerprinting.

Basic Information

Product Name	Ethidium Bromide
CAS No.	1239-45-8
Molecular Formula	C21H20BrN3
Molecular Weight	394.31 g/mol
Synonyms	3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; Homidium bromide; EtBr; Ethidium; 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; Novidium; RD 1572
EINECS	214-984-6

Quality Control

Our Ethidium Bromide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance profile verification, and loss on drying. We provide Certificates of Analysis (COA) with each shipment, ensuring traceability and compliance with research-grade specifications. Our quality commitment supports applications requiring high performance and reproducibility.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark red to brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorbance Ratio (A280/A260)	≤ 0.30 (in H2O)
Loss on Drying	≤ 1.0 %
Solubility	Soluble in water (50 mg/mL)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.