Description

2-Hydroxyestradiol 2-methyl-13C,d3-ether, 2-Methoxy-13C,d3-estradiol, 3,17β-Dihydroxy-2-methoxy-13C,d3-1,3,5(10)-estratriene, 2,3,17β-Trihydroxy-1,3,5(10)-estratriene-2-methyl-13C,d3-ether, 1,3,5(10)-Estratriene-2,3,17-triol 2-methyl-13C,d3-ether is a high-purity, stable isotope-labeled derivative of the endogenous estrogen metabolite 2-hydroxyestradiol. This compound is specifically designed as an internal standard for mass spectrometry, enabling precise quantification of estrogen metabolites in complex biological matrices. It is an essential tool for researchers and laboratories in pharmaceutical development, clinical diagnostics, and endocrinology who require the highest level of accuracy and reproducibility in their analytical workflows.

Application

• Internal Standard for LC-MS/MS: Primary application for the quantitative analysis of 2-hydroxyestradiol and related metabolites in plasma, serum, and tissue samples.
• Biomarker Research: Used in studies investigating estrogen metabolism pathways, oxidative stress, and their links to hormone-dependent cancers and other diseases.
• Drug Metabolism and Pharmacokinetics (DMPK): Critical for tracing the metabolic fate of estrogen-based pharmaceuticals and novel drug candidates.
• Clinical Mass Spectrometry: Enables the development and validation of high-sensitivity clinical assays for hormonal profiling.
• Endocrinology and Toxicology Studies: Supports research into endocrine disruption, environmental estrogens, and metabolic disorders.
• Reference Material: Serves as a certified reference standard for method calibration and quality control in analytical laboratories.

Basic Information

Product Name	2-Hydroxyestradiol 2-methyl-13C,d3-ether, 2-Methoxy-13C,d3-estradiol, 3,17β-Dihydroxy-2-methoxy-13C,d3-1,3,5(10)-estratriene, 2,3,17β-Trihydroxy-1,3,5(10)-estratriene-2-methyl-13C,d3-ether, 1,3,5(10)-Estratriene-2,3,17-triol 2-methyl-13C,d3-ether
CAS No.	1217470-09-1
Molecular Formula	C19H2413CD3O3 *
Molecular Weight	Contact for details
Synonyms	2-Methoxy-13C,d3-estradiol; 2-Hydroxyestradiol 2-methyl-13C,d3-methyl ether; 2-OCH3-13C,d3-E2; [2-13C,2,4,16,16,17-d5]-2-Methoxyestradiol; 2-MeO-13C,d3-E2; 3,17β-Dihydroxy-2-(methyl-13C,d3-oxy)-1,3,5(10)-estratriene; Estra-1,3,5(10)-triene-2,3,17-triol 2-methyl-13C,d3-ether
EINECS	Contact for details

* Formula indicates isotopic labeling at the 2-methoxy group (13C and three deuterium atoms).

Quality Control

Every batch of this stable isotope-labeled standard is produced and analyzed under strict quality control protocols to ensure isotopic purity, chemical purity, and stability. Our products undergo rigorous testing, including NMR, HPLC, and high-resolution mass spectrometry, to confirm identity and isotopic enrichment. Certificates of Analysis (COA) are provided with each shipment, detailing batch-specific analytical results. We adhere to ISO 9001 quality management principles to guarantee supply consistency and reliability for your critical research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Purity	≥ 99 atom % 13C; ≥ 98 atom % D
Chemical Purity	≥ 95%
Solubility	Soluble in methanol, ethanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.