Description

2,4(1H,3H)-Pyrimidinedione, 1-[2,5-anhydro-4-C-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-6-deoxy-α-L-mannofuranosyl]-5-methyl- is a sophisticated nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. This compound is engineered for high coupling efficiency and purity, which is paramount for ensuring the fidelity and yield of synthetic DNA and RNA sequences. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in antisense therapy, siRNA development, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer in solid-phase automated synthesizers for the production of custom DNA and RNA strands.
• Therapeutic Nucleic Acid Development: Used in the research and GMP-scale production of antisense oligonucleotides, siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Manufacturing: A critical component for synthesizing labeled probes and primers used in PCR, qPCR, and next-generation sequencing (NGS) applications.
• Genomic Research: Enables the synthesis of high-purity oligonucleotides for gene editing (e.g., CRISPR guide RNA), gene synthesis, and functional genomics studies.
• Bioconjugation and Labeling: The reactive phosphoramidite group allows for the site-specific introduction of modifications, dyes, or linkers into oligonucleotide chains.

Basic Information

Item	Detail
Product Name	2,4(1H,3H)-Pyrimidinedione, 1-[2,5-anhydro-4-C-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-6-deoxy-α-L-mannofuranosyl]-5-methyl-
CAS No.	1197033-22-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5-Methyluridine Phosphoramidite; 5-Me-dU Phosphoramidite; 1-(2,5-Anhydro-4-C-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-6-deoxy-α-L-mannofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione; 5-Methyl-2'-deoxyuridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-5-Me-dU-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of this high-value phosphoramidite is produced and tested under strict quality management systems to ensure consistency and reliability for automated synthesis. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing critical parameters such as purity (by HPLC), moisture content (KF), and identity confirmation (NMR, MS). Our quality protocols are designed to meet the exacting standards required for pharmaceutical research and GMP manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.