Description

Fmoc-Glu-Amc CAS NO 957311-37-4 is a protected amino acid derivative, specifically an Fmoc-protected glutamic acid linked to an aminocoumarin (Amc) fluorophore. This compound is a critical building block in solid-phase peptide synthesis (SPPS) and serves as a key substrate for the development of sensitive fluorogenic protease assays. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic manufacturers engaged in enzymology, high-throughput screening, and advanced peptide chemistry. The product offers high purity and reliable performance for demanding synthetic and analytical applications.

Application

• Fluorogenic Protease Substrate: Used as a sensitive substrate for assaying the activity of proteases like glutamyl endopeptidases, where cleavage releases the fluorescent aminocoumarin group.
• Solid-Phase Peptide Synthesis (SPPS): Serves as a protected glutamic acid residue for the incorporation into custom peptides, with the Fmoc group allowing for orthogonal deprotection.
• Enzyme Kinetics & High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify and characterize inhibitors of specific proteolytic enzymes.
• Biochemical Research: Utilized in academic and industrial labs for studying enzyme mechanisms, specificity, and catalytic efficiency.
• Diagnostic Reagent Development: Acts as a core component in developing fluorescent diagnostic kits for detecting protease activity in clinical or research samples.
• Peptide Library Construction: Used in the synthesis of diverse peptide libraries for screening purposes, where the glutamic acid side chain functionality is required.

Basic Information

Product Name	Fmoc-Glu-Amc
CAS No.	957311-37-4
Molecular Formula	C29H26N2O8
Molecular Weight	530.53 g/mol
Synonyms	Fmoc-Glu(AMC); N-α-Fmoc-L-glutamic acid α-(7-amido-4-methylcoumarin); Fmoc-L-glutamic acid 5-(7-amido-4-methylcoumarin); Fmoc-Glu-AMC; (9H-Fluoren-9-yl)methyl (S)-(4-((2-amino-4-methyl-2H-chromen-7-yl)amino)-4-oxobutanoyl)carbamate; Fmoc-Glutamic Acid-AMC; Fmoc-Glu-7-amido-4-methylcoumarin; Fluorogenic Glutamic Acid Substrate
EINECS	Contact for details

Quality Control

Our Fmoc-Glu-Amc is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment, ensuring compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.