Description

Biotin-Peg-Azide CAS NO 956748-40-6 is a heterobifunctional crosslinker that combines the high-affinity biotin ligand with a polyethylene glycol (PEG) spacer and a terminal azide group. This molecular architecture is critical for enabling bioorthogonal click chemistry, specifically the copper-catalyzed azide-alkyne cycloaddition (CuAAC), for precise bioconjugation and labeling applications. It is an essential reagent for researchers and developers in biopharmaceuticals, diagnostic assay development, and advanced materials science who require site-specific modification of biomolecules with minimal interference to their native function.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads or fluorescent probes to monoclonal antibodies via engineered alkyne tags.
• Diagnostic Probe Labeling: Functionalization of proteins, peptides, or oligonucleotides for use in ELISA, lateral flow assays, or fluorescence in situ hybridization (FISH).
• Cell Surface Engineering: Metabolic labeling and subsequent visualization or isolation of glycans or other cell surface components using alkyne-modified metabolic precursors.
• Affinity Chromatography Ligand Immobilization: Covalent attachment of biotinylated capture molecules to alkyne-functionalized solid supports (e.g., beads, resins, chips).
• Polymer and Biomaterial Functionalization: Introduction of biotin handles onto alkyne-containing hydrogels, nanoparticles, or other polymeric materials for downstream streptavidin-based assembly.
• Protein-Protein Interaction Studies: Crosslinking interacting partners for pull-down assays or structural analysis via click chemistry.

Basic Information

Product Name	Biotin-Peg-Azide
CAS No.	956748-40-6
Molecular Formula	C₂₈H₅₁N₇O₈S
Molecular Weight	645.8 g/mol
Synonyms	Biotin-PEG3-Azide; Biotin-PEG₃-N₃; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)pentanamide; Biotin-azide-PEG; Biotinylated PEG Azide; N-(Azido-PEG3)-biotin; Biotin-PEG-Azide crosslinker
EINECS	Contact for details

Quality Control

Our Biotin-Peg-Azide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical characterization to ensure identity, purity, and functional integrity. Certificates of Analysis (COA) are provided, detailing results from HPLC, NMR, and mass spectrometry. We support development under GMP-like conditions for applications requiring the highest traceability and documentation standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity (Click Assay)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.