Description

Biotin-Peg3-N3 CAS NO 945633-30-7 is a high-purity, heterobifunctional crosslinking reagent featuring a biotin moiety for affinity capture, a triethylene glycol (PEG3) spacer for enhanced solubility, and an azide (N3) group for bioorthogonal click chemistry. This compound is essential for researchers and developers requiring precise, stable, and efficient conjugation of biomolecules, such as proteins, antibodies, or oligonucleotides, to solid supports or detection systems. It is a critical tool for professionals in bioconjugation, proteomics, diagnostic assay development, and targeted drug delivery.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of proteins, antibodies, and peptides via copper-free click chemistry with alkyne-functionalized partners.
• Affinity Chromatography Probe Development: Immobilization onto streptavidin/avidin resins or surfaces for the capture and purification of biomolecules.
• Diagnostic Assay Fabrication: Conjugation to detection molecules (e.g., enzymes, fluorophores) for use in ELISA, lateral flow assays, or other immunoassays.
• Cell Surface Engineering: Labeling of cell membrane proteins for imaging, tracking, or targeted delivery studies.
• Polymer and Material Science: Functionalization of nanoparticles, hydrogels, or other polymeric materials to create bioactive surfaces.
• Proteomics Research: As a reagent for pull-down assays to isolate and identify protein complexes or post-translational modifications.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Used in the research and development of novel linker-payload constructs for targeted cancer therapies.

Basic Information

Item	Detail
Product Name	Biotin-Peg3-N3
CAS No.	945633-30-7
Molecular Formula	C₂₀H₃₄N₆O₆S
Molecular Weight	486.59 g/mol
Synonyms	Biotin-PEG3-azide; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-azidoethoxy)ethoxy)ethyl)pentanamide; Biotin-triethylene glycol-azide; N-(2-(2-(2-Azidoethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide; Azido-PEG3-biotin; Biotin-PEG3-N₃; Biotinylated PEG azide
EINECS	Contact for details

Quality Control

Our Biotin-Peg3-N3 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional group analysis. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to ensure reproducibility and reliability for your critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.