Description

Spdp-Dpeg(R)12-Nhs Ester CAS NO 924280-65-9 is a heterobifunctional crosslinker featuring an NHS ester and a pyridyldithiol (SPDP) group, connected by a discrete polyethylene glycol (dPEG®) spacer. This compound is engineered to facilitate the stable conjugation of biomolecules, such as antibodies and proteins, while significantly improving solubility and reducing aggregation. It is an essential reagent for researchers and manufacturers in bioconjugation, diagnostic assay development, and ADC (Antibody-Drug Conjugate) linker-payload synthesis.

Application

• Antibody-Drug Conjugate (ADC) Linker Technology: Used to attach cytotoxic payloads to monoclonal antibodies via a cleavable disulfide bond.
• Protein/Peptide Labeling: Enables site-specific modification of lysine residues with fluorescent dyes, enzymes, or other probes for imaging and detection.
• Diagnostic Reagent Conjugation: Critical for developing ELISA and lateral flow assay components by linking capture antibodies to solid supports or reporter molecules.
• PEGylation Reagent: The dPEG® spacer enhances the pharmacokinetic properties of therapeutic proteins by increasing hydrodynamic size and solubility.
• Surface Immobilization Chemistry: Facilitates the oriented covalent attachment of proteins to biosensor chips, microarrays, and other solid surfaces.
• Nanoparticle Functionalization: Used to conjugate targeting ligands (e.g., antibodies, peptides) to the surface of drug delivery nanoparticles.

Basic Information

Product Name	Spdp-Dpeg(R)12-Nhs Ester
CAS No.	924280-65-9
Molecular Formula	C58H100N4O24S2
Molecular Weight	~1301.6 g/mol
Synonyms	SPDP-dPEG®12-NHS Ester; N-Succinimidyl 3-(2-pyridyldithio)propionate-PEG12-acid; NHS-PEG12-SPDP; dPEG®12-SPDP-NHS ester; Succinimidyl-[(N-maleimidopropionamido)-dodecaethyleneglycol] ester SPDP derivative; 3-(2-Pyridyldithio)propionyl-PEG12-NHS; Heterobifunctional PEG crosslinker, NHS-PEG12-PDP
EINECS	Contact for details

Quality Control

Our Spdp-Dpeg(R)12-Nhs Ester is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and production. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity and functional integrity. We adhere to GMP-grade standards where applicable, ensuring suitability for diagnostic and biotherapeutic development. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Functional NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.