Description

4-Methylumbelliferyl 2,3,4-Tri-o-Benzoyl-6-o-(tert-Butyldiphenylsilyl)-β-D-Galactopyranoside is a sophisticated, protected glycoside derivative specifically designed as a critical substrate for enzymatic assays. This compound matters as a high-purity, chromogenic reagent essential for the sensitive detection and characterization of glycosidase enzymes, particularly β-galactosidase. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in biochemical research, enzyme kinetics studies, and the development of diagnostic tools.

Application

• Biochemical Research: Used as a fluorogenic substrate for studying the activity, inhibition, and kinetics of β-galactosidase and related enzymes.
• Diagnostic Assay Development: A key component in the formulation of sensitive diagnostic kits for genetic disorders like GM1 gangliosidosis or for bacterial identification (e.g., E. coli).
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify potential glycosidase inhibitors for therapeutic applications.
• Enzyme Characterization: Essential for determining the specificity and catalytic efficiency of novel or engineered glycosidases.
• Cell Biology & Reporter Gene Assays: Serves as a substrate in assays designed to monitor gene expression where β-galactosidase is used as a reporter.
• Quality Control in Biomanufacturing: Used to test for the presence and activity of specific enzymes in bioprocessing streams.

Basic Information

Item	Details
Product Name	4-Methylumbelliferyl 2,3,4-Tri-o-Benzoyl-6-o-(tert-Butyldiphenylsilyl)-β-D-Galactopyranoside
CAS No.	920975-58-2
Molecular Formula	C56H52O12Si
Molecular Weight	921.10 g/mol
Synonyms	4-MU-2,3,4-Tri-O-benzoyl-6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside; 4-Methylumbelliferyl 6-O-TBDPS-2,3,4-tri-O-benzoyl-β-D-galactopyranoside; TBDPS-protected 4-MU-β-D-galactopyranoside derivative; Fluorogenic β-galactosidase substrate (protected); 4-MU-β-D-Galactopyranoside, 6-O-(tert-Butyldiphenylsilyl)-2,3,4-tri-O-benzoate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 2,3,4-Tri-o-Benzoyl-6-o-(tert-Butyldiphenylsilyl)-β-D-Galactopyranoside is manufactured under strict quality protocols to ensure the high purity and batch-to-batch consistency required for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere or desiccant. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetone, and chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.