Description

Alkyne Nhs Ester (Hexynoic Acid Nhs Ester) is a high-purity, functionalized chemical reagent designed for advanced bioconjugation and labeling applications. Its core value lies in the combination of an activated NHS ester for efficient amine coupling and an alkyne handle for subsequent copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. This bifunctional nature makes it an essential building block for researchers and manufacturers in the life sciences sector, enabling the precise modification of proteins, peptides, antibodies, and other biomolecules. It is particularly critical for developing diagnostic assays, targeted drug conjugates, and advanced materials.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, enzymes, and other proteins for diagnostic and therapeutic development.
• Proteomics Research: Functionalization of biomolecules for mass spectrometry analysis, activity-based protein profiling (ABPP), and pull-down assays.
• Click Chemistry Reagent: Key intermediate for CuAAC reactions to attach fluorophores, biotin, drugs, or other functional tags to alkyne-modified substrates.
• Surface Functionalization: Modification of nanoparticles, microarrays, biosensors, and polymeric materials to create bioactive surfaces.
• Antibody-Drug Conjugate (ADC) Synthesis: Enables the construction of linker-payload complexes for targeted cancer therapies.
• Small Molecule Probe Development: Synthesis of chemical probes for studying biological pathways and target engagement.

Basic Information

Product Name	Alkyne Nhs Ester (Hexynoic Acid Nhs Ester)
CAS No.	906564-59-8
Molecular Formula	C11H13NO4
Molecular Weight	223.23 g/mol
Synonyms	5-Hexynoic Acid N-Hydroxysuccinimide Ester; 6-Heptynoic Acid, N-Hydroxysuccinimide Ester; 5-Hexynoic Acid NHS Ester; N-(5-Hexynoyloxy)succinimide; Alkyne-NHS; Alkyne-Activated Ester; Click Chemistry NHS Ester; Propargyl NHS Ester (common name for related structures); Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Our Alkyne Nhs Ester is manufactured under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and MS for identity verification, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment. We support compliance with relevant quality frameworks for research and pre-clinical manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 97%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.