Description

Cholesterol-4-13C is a stable isotope-labeled analog of cholesterol, where a carbon-13 atom is incorporated at the 4-position of the sterol backbone. This specific labeling is crucial for advanced analytical and metabolic research, providing a non-radioactive tracer with high detectability. It is an essential tool for scientists in pharmaceutical development, clinical diagnostics, and metabolic pathway analysis who require precise tracking and quantification of cholesterol and its derivatives. The compound is supplied to meet the stringent requirements of research and quality control laboratories.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative reference in LC-MS and GC-MS analyses for accurate measurement of endogenous cholesterol levels in biological samples.
• Metabolic Flux Analysis: Enables the tracing of cholesterol biosynthesis, absorption, and turnover in cell cultures, tissues, and animal models to study lipid metabolism disorders.
• Drug Metabolism and Pharmacokinetics (DMPK): Serves as a tracer to study the metabolic fate of cholesterol-lowering drugs and their effects on cholesterol synthesis pathways.
• Clinical Research and Diagnostics: Facilitates the development and validation of high-precision diagnostic assays for conditions like hypercholesterolemia and cardiovascular disease.
• Biochemical Research: Employed in enzymatic studies to investigate the mechanisms of cholesterol-modifying enzymes and transporters.
• Quality Control Reference Material: Acts as a certified reference material (CRM) for calibrating analytical instruments and validating methods in pharmaceutical and food testing laboratories.

Basic Information

Product Name	Cholesterol-4-13C
CAS No.	99964-70-2
Molecular Formula	C₂₇H₄₆¹³CO
Molecular Weight	387.66 g/mol (for ¹³C₁ isotopologue)
Synonyms	(3β)-Cholest-5-en-3-ol-4-13C; 5-Cholesten-3β-ol-4-13C; Cholesterol (4-13C); 13C Labeled Cholesterol (4-position); [4-13C]-Cholesterol; 1,2-Cyclopentanophenanthren-3-ol-4-13C derivative; Isotopic Cholesterol
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-4-13C is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing including NMR and Mass Spectrometry to confirm the position-specific 13C enrichment and chemical identity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, isotopic enrichment, and analytical results. Our quality commitment ensures the material is suitable for sensitive research and regulatory submission support.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label or COA. For long-term storage, keep the container under an inert atmosphere in a freezer (-20°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (NMR/MS)	≥ 99 atom % 13C
Chemical Purity (Titration)	≥ 97.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.