Description

n-4-Nitrobenzo-2-Oxa-1,3-Diazoledipalmitoyl Phosphatidylethanolamine is a specialized fluorescent-labeled phospholipid derivative, where the NBD (4-nitrobenzo-2-oxa-1,3-diazole) fluorophore is covalently attached to the headgroup of dipalmitoyl phosphatidylethanolamine (DPPE). This compound is critical for advanced biophysical and cell biology research, enabling the visualization and tracking of membrane dynamics, lipid trafficking, and vesicle fusion in model and cellular systems. It is an essential tool for researchers in pharmaceutical development, biochemistry, and nanotechnology who require high-purity, well-characterized probes for fluorescence-based assays and imaging.

Application

• Membrane Biophysics Research: As a fluorescent probe to study lipid bilayer properties, phase transitions, and membrane fluidity in liposomes and supported lipid bilayers.
• Cellular Imaging & Trafficking: To label cellular membranes and trace intracellular lipid transport, endocytosis, and exocytosis pathways using fluorescence microscopy.
• Liposome & Drug Delivery System Characterization: To fluorescently tag liposomal formulations, allowing for the monitoring of stability, cellular uptake, and biodistribution in delivery vehicle development.
• Protein-Lipid Interaction Studies: Used in fluorescence quenching or FRET (Förster Resonance Energy Transfer) assays to investigate the binding and activity of peptides, proteins, or drugs with lipid membranes.
• Model Membrane Assembly: A key component in constructing artificial membranes for sensor development and diagnostic assay platforms.
• Academic & Industrial R&D: Serves as a vital reagent in research laboratories within universities, pharmaceutical companies, and biotech firms focused on lipidomics and membrane science.

Basic Information

Product Name	n-4-Nitrobenzo-2-Oxa-1,3-Diazoledipalmitoyl Phosphatidylethanolamine
CAS No.	99684-86-3
Molecular Formula	C51H90N4O11P
Molecular Weight	966.25 g/mol
Synonyms	NBD-DPPE; 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl); N-(7-Nitro-2,1,3-benzoxadiazol-4-yl)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; Fluorescein DPPE; NBD Phosphatidylethanolamine; NBD PE; NBD-labeled DPPE; 4-Nitrobenzo-2-oxa-1,3-diazole Dipalmitoyl Phosphatidylethanolamine
EINECS	Contact for details

Quality Control

Our n-4-Nitrobenzo-2-Oxa-1,3-Diazoledipalmitoyl Phosphatidylethanolamine is produced and handled under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic confirmation of structure and labeling. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profile is supplied with every shipment, supporting compliance with rigorous research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to warm to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 98.0%
Extinction Coefficient	~21,000 M-1cm-1 at ~460 nm in methanol
Solubility	Soluble in chloroform, methanol, DMSO; forms micelles in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.