Description

H-Glu-Ala-Pna is a high-purity synthetic peptide derivative, specifically a chromogenic substrate designed for enzymatic research and diagnostic applications. Its core value lies in its role as a specific and sensitive substrate for proteolytic enzymes, enabling precise biochemical analysis and assay development. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and in-vitro diagnostic (IVD) kit production sectors, where reliable enzyme activity measurement is critical.

Application

• Enzyme Activity Assays: Primary substrate for measuring the activity of specific proteases, including aminopeptidases and related enzymes in research settings.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibitor screening to understand proteolytic pathways.
• Diagnostic Reagent Manufacturing: A key component in the formulation of chromogenic reagents for clinical diagnostic kits, particularly for detecting enzyme markers.
• Pharmaceutical Development: Employed in drug discovery pipelines for high-throughput screening (HTS) of compounds targeting specific proteases.
• Quality Control in Bioprocessing: Monitors enzyme purity and activity during the production of therapeutic proteins and other biologics.

Basic Information

Item	Detail
Product Name	H-Glu-Ala-Pna
CAS No.	99524-10-4
Molecular Formula	C17H23N5O6
Molecular Weight	393.40 g/mol
Synonyms	L-Glutamyl-L-alanine 4-nitroanilide; Glu-Ala-pNA; H-Glu-Ala-pNA; L-γ-Glutamyl-L-alanine p-nitroanilide; N-(L-γ-Glutamyl)-L-alanine 4-nitroanilide; Substrate for γ-Glutamyltransferase; GGT Substrate; Peptide chromogenic substrate 99524-10-4
EINECS	Contact for details

Quality Control

Our H-Glu-Ala-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our clients in GMP-compliant manufacturing and research.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.