Description

Holo-Transferrin is a high-purity, iron-saturated form of the glycoprotein transferrin, essential for iron transport and metabolism in biological systems. This product is critical for research and development in life sciences, where consistent iron-binding capacity and structural integrity are paramount. It is primarily utilized by the biopharmaceutical, cell culture, and diagnostic industries for applications requiring a defined and bioavailable iron source.

Application

• Cell Culture Media Supplement: Provides a controlled source of bioavailable iron for the growth of mammalian cells, including hybridomas and CHO cells.
• Biopharmaceutical Research: Used in the study of iron metabolism, drug delivery systems, and the development of protein-based therapeutics.
• Diagnostic Reagent Manufacturing: Serves as a key component in immunoassays and clinical chemistry tests for measuring iron status.
• Neuroscience & Biochemistry Studies: Employed in research on the blood-brain barrier, neuronal iron uptake, and oxidative stress mechanisms.
• Standard & Reference Material: Acts as a calibrated standard for analytical methods such as HPLC, electrophoresis, and mass spectrometry.
• Tissue Engineering: Incorporated into scaffolds and growth matrices to support cell proliferation and differentiation.

Basic Information

Product Name	Holo-Transferrin
CAS No.	11096-37-0
Molecular Formula	C1865H3019N515O583S12 (Approximate, for human transferrin)
Molecular Weight	~79.5 kDa
Synonyms	Iron-saturated Transferrin; Iron(III)-Transferrin; Apo-transferrin (iron-bound form); Siderophilin; Tf-Fe; Transferrin, Holo; Serotransferrin (iron-saturated); β-1-Metal-binding Globulin; Tf(Fe2)3+
EINECS	234-318-9

Quality Control

Our Holo-Transferrin is manufactured under strict quality management systems. Each batch is tested to ensure high purity, correct iron saturation, and functional activity. Certificates of Analysis (COA) are provided, detailing key parameters such as purity (by HPLC), iron content, endotoxin levels, and biological activity. We support compliance with relevant guidelines for research and bioprocessing applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or inert atmosphere to maintain stability. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Reddish-brown to brown powder or lyophilized solid
Identification (IR)	Conforms to standard
Purity (HPLC)	≥ 95%
Iron Saturation	> 95%
Protein Content (Biuret)	≥ 96%
Endotoxin	< 1.0 EU/mg
Solubility	Soluble in water or buffer (PBS, pH ~7.4)
Biological Activity	Conforms to cell culture performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.