Description

Nalpha-(tert-Butoxycarbonyl)-L-Arginine 4-Nitroanilide Hydrochloride is a protected arginine derivative, specifically designed as a chromogenic substrate for the study and detection of proteolytic enzymes. This compound is critical for researchers and manufacturers requiring high-purity, reliable biochemical tools for enzyme kinetics and diagnostic assay development. It is primarily utilized in pharmaceutical research, biochemical diagnostics, and academic laboratories focused on protease activity and inhibition studies.

Application

• Protease Substrate: Acts as a specific chromogenic substrate for trypsin-like serine proteases, enabling the spectrophotometric measurement of enzyme activity.
• Biochemical Research: Used in kinetic studies to determine enzyme inhibition constants (Ki) and catalytic efficiency for drug discovery programs.
• Diagnostic Assay Development: Employed in the formulation of in-vitro diagnostic (IVD) kits for detecting and quantifying protease levels in clinical samples.
• Pharmaceutical Quality Control: Serves as a reference standard in the quality testing of therapeutic enzymes and related biopharmaceutical products.
• Cell Biology Studies: Utilized to monitor and characterize protease activity in cell culture and tissue extract models.

Basic Information

Product Name	Nalpha-(tert-Butoxycarbonyl)-L-Arginine 4-Nitroanilide Hydrochloride
CAS No.	99306-64-6
Molecular Formula	C17H26N6O5 • HCl
Molecular Weight	446.89 g/mol
Synonyms	Boc-Arg-pNA • HCl; Nα-Boc-L-arginine 4-nitroanilide hydrochloride; BOC-L-Arg-pNA hydrochloride; L-Arginine, N2-[(1,1-dimethylethoxy)carbonyl]-, 4-nitroanilide, monohydrochloride; Nalpha-(tert-Butoxycarbonyl)-L-arginine p-nitroanilide hydrochloride; BOC-Arg-4-NA • HCl; Nα-(tert-Butoxycarbonyl)-L-arginine 4-nitroanilide hydrochloride; Substrate for Trypsin
EINECS	Contact for details

Quality Control

Our Nalpha-(tert-Butoxycarbonyl)-L-Arginine 4-Nitroanilide Hydrochloride is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	97.0% - 103.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Specific Rotation	-10.0° to -14.0° (c=1 in DMF)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.