Description

n-Benzoyl-Dl-Arginine-4-Nitroanilide Hydrochloride is a chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the specific and sensitive detection of trypsin-like proteolytic activity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in protease inhibitor screening and enzyme kinetics studies. The product is supplied as a high-purity reagent to ensure reliable and reproducible experimental results.

Application

• Protease Activity Assays: As a specific chromogenic substrate for the detection and quantification of trypsin, thrombin, plasmin, and other serine proteases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for pancreatic function testing and coagulation disorders.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: Essential for studying enzyme kinetics, inhibition constants (Ki), and catalytic mechanisms of proteolytic enzymes in academic and industrial laboratories.
• Quality Control in Biotechnology: Employed to assess the purity and specific activity of enzyme preparations, including recombinant proteases.
• Food Science & Microbiology: Applied in research to analyze proteolytic activity in food processing and microbial enzyme characterization.

Basic Information

Product Name	n-Benzoyl-Dl-Arginine-4-Nitroanilide Hydrochloride
CAS No.	911-77-3
Molecular Formula	C20H25N7O4 • HCl
Molecular Weight	464.92 g/mol
Synonyms	BAPNA; Bz-DL-Arg-pNA • HCl; N-α-Benzoyl-DL-arginine 4-nitroanilide hydrochloride; Benzoyl-DL-arginine-p-nitroanilide hydrochloride; DL-BAPA; N-Benzoyl-DL-arginine-4-nitroanilide HCl; Bz-Arg-pNA • HCl; Trypsin substrate BAPNA
EINECS	213-027-2

Quality Control

Our n-Benzoyl-Dl-Arginine-4-Nitroanilide Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by IR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing assay, impurities, and physical characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, yellow solution in DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.