Description

(L)-Methionyl-(L)-Prolyl-p-Nitroanilide is a specialized synthetic peptide substrate designed for enzymatic research and diagnostic applications. This compound is critical for the precise measurement of proteolytic enzyme activity, particularly in biochemical assays. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology and protease inhibitor studies.

Application

• Protease Activity Assays: As a chromogenic substrate for the kinetic study of various proteases, including dipeptidyl peptidase IV (DPP-IV) and related enzymes.
• Pharmaceutical Research: Used in high-throughput screening (HTS) for the discovery and development of novel protease inhibitors as potential drug candidates.
• Clinical Diagnostics: A key component in enzymatic assay kits for the determination of specific enzyme levels in biological samples for disease diagnosis.
• Biochemical Research: Employed in mechanistic studies to understand enzyme kinetics, substrate specificity, and inhibition profiles.
• Quality Control: Used in the biomanufacturing sector for the potency testing of enzyme-based biopharmaceuticals.

Basic Information

Product Name	(L)-Methionyl-(L)-Prolyl-p-Nitroanilide
CAS No.	99264-69-4
Molecular Formula	C19H27N5O5S
Molecular Weight	437.51 g/mol
Synonyms	Methionyl-Prolyl-p-Nitroanilide; Met-Pro-pNA; DPP-IV Substrate; H-Met-Pro-pNA; L-Methionyl-L-prolyl 4-nitroanilide; Dipeptidyl Peptidase IV Substrate; 4-Nitroanilide of Methionylproline; H-Met-Pro-4-nitroanilide
EINECS	Contact for details

Quality Control

Our (L)-Methionyl-(L)-Prolyl-p-Nitroanilide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for fine chemicals and can provide material tailored to specific research-grade or diagnostic-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in DMSO or DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.