Description

5-Hexyl-2,3-Diphenyl-2H-Tetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical applications. Its core value lies in its role as a redox indicator and electron acceptor in cell viability and cytotoxicity assays, providing critical data for research and development. This compound is essential for professionals in pharmaceutical R&D, biotechnology, and academic research laboratories focused on cell biology and drug discovery. Its reliable performance makes it a key component for high-throughput screening and metabolic activity studies.

Application

• Cell Viability and Proliferation Assays: Used as a substrate in MTT-like assays to measure mitochondrial activity in living cells.
• Cytotoxicity Screening: Employed in pharmaceutical and toxicological research to assess the toxic effects of chemical compounds on cell cultures.
• Microbiological Analysis: Applied in microbial viability tests and for monitoring bacterial dehydrogenase activity.
• Biochemical Research: Serves as an electron acceptor in studies of enzyme kinetics, particularly for dehydrogenases.
• Drug Discovery Platforms: Integral to high-throughput screening (HTS) systems for identifying potential therapeutic agents.
• Material Science & Biocompatibility Testing: Used to evaluate the cellular response to new biomaterials and medical devices.

Basic Information

Product Name	5-Hexyl-2,3-Diphenyl-2H-Tetrazolium Chloride
CAS No.	99077-15-3
Molecular Formula	C25H27ClN4
Molecular Weight	418.96 g/mol
Synonyms	Hexyl Tetrazolium Chloride; HDPT Chloride; 2,3-Diphenyl-5-hexyl-2H-tetrazolium Chloride; 5-Hexyl-2,3-diphenyltetrazolium Chloride; Tetrazolium Red Chloride (related analog); HDPT; Cell Viability Assay Salt; Dehydrogenase Indicator Salt
EINECS	Contact for details

Quality Control

Our 5-Hexyl-2,3-Diphenyl-2H-Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by FT-IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment away from direct light exposure to maintain stability and potency.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, ethanol; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.