Description

Mmp-2/Mmp-9 Substrate Ii is a specialized fluorogenic peptide substrate designed for the sensitive and selective detection of matrix metalloproteinase-2 (MMP-2, Gelatinase A) and MMP-9 (Gelatinase B) activity. This compound is critical for researchers and developers in life sciences and pharmaceutical discovery, enabling precise enzymatic assays to study cancer metastasis, tissue remodeling, and inflammatory diseases. It is an essential tool for biochemical research, high-throughput screening (HTS) for drug candidates, and diagnostic assay development in academic, biotech, and pharmaceutical laboratories.

Application

• Biochemical Research & Enzyme Kinetics: Used as a specific substrate to measure the catalytic activity and inhibition of MMP-2 and MMP-9 in purified enzyme systems.
• Drug Discovery & High-Throughput Screening (HTS): Enables the screening and evaluation of potential therapeutic inhibitors targeting gelatinases in oncology and inflammatory disease research.
• Cancer Biology Studies: Facilitates research into tumor invasion, angiogenesis, and metastasis where MMP-2 and MMP-9 are key mediators.
• Diagnostic Assay Development: Serves as a core component in developing in vitro diagnostic (IVD) or research-use-only (RUO) assays for detecting MMP activity in biological samples.
• Tissue Remodeling & Wound Healing Research: Applied in studies investigating extracellular matrix (ECM) degradation and repair processes.
• Inflammatory Disease Models: Used to monitor MMP activity in models of arthritis, atherosclerosis, and other conditions involving tissue degradation.

Basic Information

Product Name	Mmp-2/Mmp-9 Substrate Ii
CAS No.	98992-65-5
Molecular Formula	C34H46N8O10
Molecular Weight	726.78 g/mol
Synonyms	Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2; (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2; MMP-2/MMP-9 Fluorogenic Substrate; MMP-2/9 Substrate II; DQ Gelatin (a related commercial name for fluorescent gelatin, often used for similar applications); Fluorogenic Peptide Substrate for Gelatinases; Mca-PLGL-Dpa-AR-NH2
EINECS	Contact for details

Quality Control

Our Mmp-2/Mmp-9 Substrate Ii is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and mass spectrometry (MS) for identity confirmation. We provide comprehensive Certificates of Analysis (COA) detailing purity, performance data in standard enzyme assays, and other critical specifications. Our quality system supports research requiring reliable and reproducible biochemical tools.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area Percent)
Water Content (KF)	≤ 5.0 %
Performance (Enzymatic Assay)	Cleavage by recombinant MMP-2/MMP-9 produces characteristic fluorescence increase (Km value available on COA)
Solubility	Soluble in DMSO, DMF; Limited solubility in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.