Description

12,13-Didehydro-F430 is a specialized derivative of the nickel-containing tetrapyrrole cofactor F430, identified by CAS number 98971-74-5. This compound is of significant interest in biochemical and microbiological research, particularly in the study of methanogenic archaea and the enzymatic mechanisms of methyl-coenzyme M reductase (MCR). It serves as a critical reference standard and research tool for scientists investigating anaerobic methane metabolism, enzyme catalysis, and novel biocatalytic pathways. Key users include research institutions, pharmaceutical R&D departments, and industrial biotechnology firms focused on enzyme engineering and metabolic studies.

Application

• Biochemical Research: As a reference standard and probe for studying the structure and function of methyl-coenzyme M reductase (MCR) in methanogens.
• Enzymology Studies: Investigation of catalytic mechanisms, inhibition, and the role of modified tetrapyrrole cofactors in anaerobic metabolism.
• Pharmaceutical R&D: Potential use in the development of novel antimicrobial agents targeting methanogenic pathways.
• Industrial Biotechnology: Research into engineered enzymes and biocatalytic processes for methane conversion or value-added chemical synthesis.
• Analytical Chemistry: Serves as a high-purity calibrant for advanced analytical techniques such as HPLC, LC-MS, and NMR in specialized laboratories.
• Microbiology: Tool for understanding the physiology and biochemistry of archaea and other microorganisms utilizing unique cofactors.

Basic Information

Item	Details
Product Name	12,13-Didehydro-F430
CAS No.	98971-74-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	12,13-Didehydro-F430; F430 Derivative; 12,13-Didehydrofactor 430; Nickel Tetrapyrrole Derivative; MCR Cofactor Analog; 98971-74-5; Factor 430 Derivative
EINECS	Contact for details

Quality Control

Every batch of 12,13-Didehydro-F430 is subjected to rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocols are designed to meet the exacting standards required for advanced research applications. A comprehensive Certificate of Analysis (COA), detailing results from techniques such as HPLC, NMR, and mass spectrometry, is provided with each shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (e.g., argon or nitrogen) at controlled room temperature (15-25°C) or as specified on the label or COA. This product is light-sensitive and easily oxidized; prolonged exposure to air, light, or moisture should be avoided to maintain stability.

Specification

Item	Specification
Appearance	Solid (Color to be specified)
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Research Grade)
Related Substances (HPLC)	≤ 5.0% total
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.