Description

Dmt-Dt Phosphoramidite CAS NO 98796-51-1 is a high-purity, protected nucleoside phosphoramidite monomer essential for automated oligonucleotide synthesis. This compound is a critical building block for constructing DNA sequences with precise control and high coupling efficiency. It is primarily required by pharmaceutical R&D, biotechnology, and diagnostic industries for applications in antisense therapy, PCR primers, and genetic research.

Application

• Oligonucleotide Synthesis: A key monomer for the solid-phase synthesis of DNA strands in automated synthesizers.
• Therapeutic Development: Used in the production of antisense oligonucleotides (ASOs) and siRNA for targeted drug discovery.
• Molecular Diagnostics: Manufacture of primers and probes for PCR, qPCR, and next-generation sequencing (NGS) applications.
• Genetic Research: Synthesis of custom DNA fragments for cloning, gene editing (e.g., CRISPR), and functional genomics studies.
• Bioconjugation: Preparation of labeled oligonucleotides for imaging, sensing, and diagnostic assays.
• Academic & Industrial R&D: Fundamental reagent for nucleic acid chemistry research and development of novel biomolecules.

Basic Information

Product Name	Dmt-Dt Phosphoramidite
CAS No.	98796-51-1
Molecular Formula	C41H49N4O8P
Molecular Weight	756.83 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine 3'-[(2-Cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-dT Phosphoramidite; Thymidine Phosphoramidite, DMT-protected; dT Phosphoramidite; 2'-Deoxythymidine Phosphoramidite; Amidite dT; DNA Synthesis Monomer dT; 5'-DMT-thymidine-3'-CE-phosphoramidite
EINECS	Contact for details

Quality Control

Our Dmt-Dt Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive synthesis workflows. Each lot undergoes comprehensive analytical testing, including HPLC for purity and 31P NMR for identity and stability. A Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and easily oxidized nature, the material should be brought to room temperature in a desiccator before opening to prevent condensation and degradation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Moisture Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.