Description

H-Ile-Amc CAS NO 98516-74-6 is a specialized protected amino acid derivative, specifically H-Isoleucine-7-amido-4-methylcoumarin. This compound is a critical fluorogenic substrate used extensively in enzymatic assays and biochemical research. It serves as a key reagent for the sensitive detection and quantification of protease activity, particularly for enzymes like chymotrypsin and related serine proteases. Researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors rely on this high-purity biochemical for developing assays and studying enzyme kinetics.

Application

• Protease Activity Assays: Primary use as a fluorogenic substrate for measuring the activity of chymotrypsin, elastase, and other serine proteases in research and high-throughput screening.
• Biochemical Research: Essential tool in enzymology for kinetic studies, inhibitor screening, and characterizing protease specificity and mechanism of action.
• Diagnostic Kit Development: Used in the formulation of diagnostic kits and reagents for clinical and research laboratories to detect specific protease biomarkers.
• Pharmaceutical Development: Employed in drug discovery pipelines to identify and validate novel protease inhibitors as potential therapeutic agents.
• Quality Control in Biomanufacturing: Applied in monitoring protease activity during upstream and downstream bioprocessing of therapeutic proteins.

Basic Information

Product Name	H-Ile-Amc
CAS No.	98516-74-6
Molecular Formula	C20H26N2O4
Molecular Weight	358.44 g/mol
Synonyms	H-Isoleucine-7-amido-4-methylcoumarin; L-Isoleucyl-7-amido-4-methylcoumarin; Ile-AMC; Isoleucine-AMC; AMC-Ile; 7-Amido-4-methylcoumarin-Ile; Fluorogenic substrate for chymotrypsin; Protease substrate 98516-74-6
EINECS	Contact for details

Quality Control

Every batch of H-Ile-Amc is manufactured and tested under strict quality management systems to ensure the highest standards of purity and performance for sensitive biochemical applications. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment and handled under conditions that minimize exposure to ambient light and humidity to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.