Description

3-Phenyl-7-Coumarinyl Phosphate Hemipyridine Salt is a specialized biochemical reagent primarily used as a sensitive fluorogenic substrate for enzyme assays. This compound matters for researchers and manufacturers requiring precise detection and quantification of enzymatic activity, particularly for phosphatases and phosphodiesterases. It is needed by professionals in pharmaceutical R&D, diagnostic kit manufacturing, and academic biochemistry laboratories for high-throughput screening and mechanistic studies.

Application

• Fluorogenic Substrate for Alkaline Phosphatase (ALP): A key component in ELISA and other immunoassays for signal amplification and detection.
• Enzyme Kinetics Research: Used to study the catalytic mechanism and inhibition of various phosphatases.
• Diagnostic Reagent Manufacturing: Incorporated into clinical diagnostic kits for the detection of bone, liver, and other diseases marked by elevated ALP levels.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify potential enzyme inhibitors or activators.
• Cell Biology & Histochemistry: Used as a substrate for visualizing phosphatase activity in cells and tissue sections.
• Biosensor Development: Integrated into sensor systems for environmental monitoring or point-of-care testing.

Basic Information

Product Name	3-Phenyl-7-Coumarinyl Phosphate Hemipyridine Salt
CAS No.	97970-92-8
Molecular Formula	C24H20NO7P
Molecular Weight	465.39 g/mol
Synonyms	7-(Dihydrogen phosphate)-3-phenylcoumarin hemipyridine salt; 3-Phenylumbelliferyl phosphate hemipyridine salt; 3-Phenyl-7-hydroxycoumarin phosphate hemipyridine salt; 3-Phenyl-7-HC phosphate hemipyridine salt; PUP hemipyridine salt; 3-Phenyl-7-coumaryl phosphate hemipyridine salt; Fluorogenic phosphatase substrate; ALP substrate (3-phenylumbelliferyl phosphate)
EINECS	Contact for details

Quality Control

Our 3-Phenyl-7-Coumarinyl Phosphate Hemipyridine Salt is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorescence	Exhibits characteristic blue fluorescence upon enzymatic hydrolysis

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.