Description

5-Bromo-3-Indolyl-β-D-Galactopyranoside is a chromogenic substrate widely used in molecular biology and biochemistry for the detection of β-galactosidase enzyme activity. This compound is critical for applications such as blue-white screening in molecular cloning, reporter gene assays, and histochemical staining. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable, high-purity substrates for enzymatic analysis and product development.

Application

• Molecular Cloning (Blue-White Screening): A key component in the identification of recombinant bacterial colonies containing plasmids with inserts, where β-galactosidase activity is disrupted.
• Reporter Gene Assays: Used to measure and quantify gene expression levels in cell-based assays when linked to the lacZ reporter gene.
• Histochemistry & Cytochemistry: Employed for the localization of β-galactosidase activity in tissue sections and fixed cells, producing an insoluble blue precipitate.
• Enzyme Activity Testing: Serves as a standard substrate for the biochemical characterization and kinetic analysis of β-galactosidase enzymes.
• Diagnostic Kit Manufacturing: A vital raw material in the production of diagnostic kits and reagents for research and clinical laboratories.
• Microbiology Research: Used in studies of bacterial gene regulation and promoter activity within the lac operon system.

Basic Information

Product Name	5-Bromo-3-Indolyl-β-D-Galactopyranoside
CAS No.	97753-82-7
Molecular Formula	C14H16BrNO6
Molecular Weight	374.18 g/mol
Synonyms	X-Gal; 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (common trade name); 5-Bromo-4-chloro-3-indolyl β-D-galactoside; β-Galactosidase substrate X-Gal; 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside; BCI-Gal; Bluo-Gal; Galactoside indoxyl derivative.
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indolyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and related substance profiles are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.