Description

Superose 12 is a high-performance, cross-linked agarose-based gel filtration chromatography medium designed for high-resolution separation of biomolecules. This product is essential for precise size-exclusion chromatography (SEC) in research, analytical, and process development workflows. It is primarily used by scientists and process engineers in the biotechnology and pharmaceutical industries for the purification and analysis of proteins, peptides, nucleic acids, and other macromolecules.

Application

• High-Resolution Size-Exclusion Chromatography (SEC) for analytical and preparative separation of biomolecules.
• Protein Purification, including monoclonal antibodies, enzymes, and recombinant proteins.
• Oligonucleotide and Plasmid DNA Analysis to determine molecular size and purity.
• Desalting and Buffer Exchange of protein samples in laboratory-scale preparations.
• Quality Control Analysis for assessing aggregation states and stability of biopharmaceuticals.
• Process Development and optimization of downstream purification steps in biomanufacturing.

Basic Information

Product Name	Superose 12
CAS No.	97599-42-3
Molecular Formula	Contact for details
Molecular Weight	N/A (Chromatography resin)
Synonyms	Superose™ 12; Agarose Cross-linked Beads; SEC Medium; Gel Filtration Resin; Size Exclusion Chromatography Gel; Agarose 12; Fast Desalting Column Media; High Resolution SEC Beads
EINECS	Contact for details

Quality Control

Our Superose 12 is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for critical separations. Each lot is tested for key parameters including particle size distribution, exclusion limits, flow characteristics, and pressure stability. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store the suspension in the supplied storage solution (typically 20% ethanol) at 2-8°C (36-46°F). Do not freeze. For long-term storage, ensure the container is sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Appearance	White suspension of spherical beads
Matrix	Cross-linked agarose
Particle Size	10 - 15 μm (mean diameter)
Exclusion Limit (Globular Proteins)	~300,000 Da
Operating pH Range	2 - 13 (long term), 3 - 12 (short term)
Maximum Operating Pressure	0.3 MPa (3 bar, 43 psi)
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.