Description

4-Methylumbelliferyl Phosphocholine is a specialized fluorogenic substrate used in enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of phospholipase activity. It is primarily utilized in life science research, pharmaceutical development, and diagnostic applications where high sensitivity and specificity are paramount.

Application

• Enzymatic Substrate for Phospholipase Assays: Specifically used as a fluorogenic substrate for phospholipase C (PLC) and phospholipase D (PLD) enzymes in biochemical research.
• High-Throughput Screening (HTS): Enables rapid, sensitive detection of enzyme activity in drug discovery platforms targeting lipid signaling pathways.
• Diagnostic Kit Development: Serves as a key component in the development of clinical diagnostic assays for conditions related to phospholipase dysregulation.
• Cell Biology & Signal Transduction Studies: Used to investigate the role of phospholipases in cellular processes, membrane dynamics, and second messenger generation.
• Quality Control in Pharmaceutical Manufacturing: Employed in potency testing and stability studies of therapeutic enzymes or inhibitors.
• Academic & Industrial Research: A fundamental tool for studying enzyme kinetics, inhibitor characterization, and pathway analysis in biochemistry laboratories.

Basic Information

Product Name	4-Methylumbelliferyl Phosphocholine
CAS No.	97055-84-0
Molecular Formula	C15H23NO8P
Molecular Weight	376.32 g/mol
Synonyms	4-MU-PC; 4-Methylumbelliferyl Phosphorylcholine; 4-Methylumbelliferyl Choline Phosphate; Phosphocholine 4-methylumbelliferyl ester; 7-Hydroxy-4-methylcoumarin choline phosphate ester; 4-Methylumbelliferyl-Phosphocholine; Fluorogenic phospholipase substrate; 4-MU Phosphorylcholine
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Phosphocholine is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis and functional activity validation. Certificates of Analysis (COA) detailing purity, identity, and assay-specific performance data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (in standard PLC assay)
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.