Description

Pholasin is a highly purified, naturally derived chemiluminescent substrate isolated from the marine bivalve *Pholas dactylus*. This compound is critical for sensitive and rapid detection of reactive oxygen species (ROS) and free radicals in biological and chemical systems. It serves as an essential tool for researchers and quality control professionals in the pharmaceutical, biotechnology, and environmental monitoring industries, enabling real-time analysis of oxidative stress and enzymatic activity.

Application

• Biological Research: Detection and quantification of superoxide anion, hydrogen peroxide, and other reactive oxygen species (ROS) in cell cultures and tissue samples.
• Pharmaceutical Development: High-throughput screening of antioxidant compounds and evaluation of drug-induced oxidative stress in preclinical studies.
• Diagnostic Assays: Core component in chemiluminescence-based immunoassays and biosensors for clinical diagnostics.
• Environmental Monitoring: Measurement of pollutant-induced oxidative stress in aquatic organisms and assessment of water quality.
• Food & Beverage Analysis: Monitoring lipid peroxidation and evaluating the efficacy of natural and synthetic antioxidants in food products.
• Cosmetic Science: Testing the anti-oxidant and radical-scavenging properties of skincare and cosmetic formulations.

Basic Information

Product Name	Pholasin
CAS No.	96827-88-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pholas Dactylus Luciferin; *Pholas* Luciferin; *Pholas* Oxyluciferin; CL Substrate from *Pholas dactylus*; Marine Bioluminescent Substrate; Reactive Oxygen Species (ROS) Detection Reagent; Superoxide Anion Detection Reagent
EINECS	Contact for details

Quality Control

Our Pholasin is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance. Each lot is subjected to rigorous analytical testing, including purity assessment by HPLC and functional activity validation in standardized chemiluminescence assays. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). For long-term stability, store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature in a dark environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity (Chemiluminescence)	≥ 90% of reference standard
Solubility	Soluble in aqueous buffers, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.