Description

Ac-Ala-Ala-Pro-Ala-Pna CAS NO 96699-74-0 is a synthetic, chromogenic peptide substrate specifically designed for the study and detection of proteolytic enzyme activity. This compound is a critical tool in biochemical research and pharmaceutical development, offering high sensitivity and specificity for enzymatic assays. It is primarily utilized by researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic industries for investigating serine proteases and related enzymes.

Application

• Enzyme Kinetics Research: Used as a specific substrate to study the catalytic activity, inhibition, and kinetics of target proteases like dipeptidyl peptidase IV (DPP-IV) and related enzymes.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential therapeutic inhibitors for metabolic and inflammatory diseases.
• Diagnostic Reagent Development: Serves as a key component in developing colorimetric or fluorometric diagnostic kits for clinical testing and biomarker detection.
• Biochemical Assay Standard: Acts as a reference standard in quality control for assay validation and ensuring consistency in research and manufacturing processes.
• Cell Biology Studies: Applied in cellular assays to monitor protease activity and signaling pathways in live cells or tissue extracts.

Basic Information

Product Name	Ac-Ala-Ala-Pro-Ala-Pna
CAS No.	96699-74-0
Molecular Formula	C23H31N7O7
Molecular Weight	517.54 g/mol
Synonyms	Acetyl-Ala-Ala-Pro-Ala-p-Nitroanilide; Ac-AAPF-pNA; N-Acetyl-Alanyl-Alanyl-Prolyl-Alanyl-4-Nitroanilide; Ala-Ala-Pro-Ala-pNA (acetylated); DPP-IV Substrate; Chromogenic Peptide Substrate; Protease Substrate IV; Ac-AAPF-pNA
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Pro-Ala-Pna is manufactured under strict quality management systems. Every batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) providing batch-specific data for identity, purity, and assay is supplied with each order to guarantee traceability and reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.