Description

Leucine, L-[3,4,5-3H(N)]- is a high-specific-activity, tritium-labeled isotopologue of the essential amino acid L-Leucine. This radiolabeled compound is a critical tool for metabolic and pharmacokinetic research, enabling precise tracking and quantification in biological systems. It is primarily utilized by researchers in pharmaceutical development, biochemistry, and nutritional science for tracer studies, receptor binding assays, and ADME (Absorption, Distribution, Metabolism, and Excretion) profiling.

Application

• Biomedical Tracer Studies: Used to investigate protein synthesis rates, amino acid transport mechanisms, and metabolic pathways in cell cultures and animal models.
• Drug Metabolism & Pharmacokinetics (DMPK): Essential for conducting radiolabeled ADME studies to determine the fate of new chemical entities in the body.
• Receptor-Ligand Binding Assays: Serves as a high-affinity tracer for studying leucine-specific receptors or transporters.
• Nutritional Research: Tracks the utilization and turnover of branched-chain amino acids (BCAAs) in muscle metabolism studies.
• Biochemical Pathway Analysis: Elucidates the catabolic and anabolic routes of leucine, including its role in the mTOR signaling pathway.
• Quality Control Reference: Can be used as an internal standard in advanced analytical techniques like LC-MS for method development and validation.

Basic Information

Product Name	Leucine, L-[3,4,5-3H(N)]-
CAS No.	96084-09-2
Molecular Formula	C6H113H3NO2 *
Molecular Weight	~136.18 g/mol (for unlabeled); Actual MW varies with specific activity.
Synonyms	(2S)-2-Amino-4-methyl-3,4,5-tritritiovaleric Acid; L-[3,4,5-3H]Leucine; Tritiated L-Leucine; [3,4,5-3H(N)]-L-Leucine; (S)-2-Amino-4-methyl-3,4,5-t3H-pentanoic Acid; H-3 Leucine; L-Leucine-t3
EINECS	Contact for details

* Formula indicates tritium (³H) labeling at the 3, 4, and 5 positions of the leucine molecule.

Quality Control

Every batch of Leucine, L-[3,4,5-3H(N)]- is manufactured and analyzed under strict quality systems. We provide comprehensive analytical documentation to support your research. Certificates of Analysis (COA) are supplied with each shipment, detailing critical parameters such as radiochemical purity, specific activity, chemical purity, and solvent composition. Our production adheres to GMP-like guidelines for radiochemicals, ensuring consistency and reliability for your long-term studies.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep in a dedicated, properly shielded freezer. Users must follow all local and international regulations for the safe handling, storage, and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Specific Activity	60 - 120 Ci/mmol (Contact for exact value)
Radiochemical Purity (RCP)	≥ 95% (by HPLC)
Chemical Purity (HPLC)	≥ 98%
Concentration	1 mCi/mL (in ethanol:water mixture)
Identification	Co-chromatography with reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.