Description

Lipid A, Diphosphoryl From Escherichia Coli F-583 (Rd Mutant) is a purified, diphosphorylated lipid A derivative isolated from the rough mutant strain *E. coli* F-583 (Rd). This compound serves as a critical potent agonist for Toll-like receptor 4 (TLR4) and is a fundamental building block for studying innate immune responses. It is essential for researchers and developers in immunology, vaccine adjuvant research, and pharmaceutical development requiring a well-defined endotoxin component.

Application

• Immunological Research: As a standard TLR4 ligand for studying innate immune signaling pathways, cytokine induction, and macrophage activation.
• Vaccine Adjuvant Development: Investigation and formulation of novel adjuvants to enhance vaccine efficacy.
• Endotoxin Structure-Activity Relationship (SAR) Studies: Used as a reference compound to understand the molecular basis of lipid A bioactivity.
• Cell-Based Assays: Stimulation agent in cell culture models to induce inflammatory responses for drug screening.
• Quality Control & Standardization: Serves as a reference standard in the quality control of biological products and in LAL (Limulus Amebocyte Lysate) assay validation.
• Biochemical Reagent: Key component in reconstitution experiments for studying membrane protein complexes and lipid biochemistry.

Basic Information

Product Name	Lipid A, Diphosphoryl From Escherichia Coli F-583 (Rd Mutant)
CAS No.	95991-05-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Diphosphoryl Lipid A (E. coli F583); Lipid A, Diphosphoryl (Escherichia coli F583 Rd mutant); E. coli F583 Rd Mutant Lipid A; TLR4 Agonist Lipid A; Bacterial Endotoxin Lipid A; Monophosphoryl Lipid A precursor; Hexa-acylated Lipid A; LA-15-PP
EINECS	Contact for details

Quality Control

Our Lipid A, Diphosphoryl is produced and handled under stringent conditions to ensure batch-to-batch consistency and biological activity. Quality is verified through a suite of analytical techniques including HPLC, mass spectrometry, and endotoxin unit quantification. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and biological activity is provided with each batch to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This material is easily oxidized; aliquoting under an inert atmosphere is recommended for extended use. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Endotoxin Activity (LAL)	≥ 1.0 x 10⁶ EU/mg
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in chloroform/methanol/water mixtures

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.