Description

2-(Biotinylamido)Ethanol is a biotinylated derivatizing reagent used to introduce biotin functionality onto hydroxyl-containing molecules via stable amide bond formation. It serves as a critical linker in affinity-based detection, purification, and immobilization workflows where high-affinity streptavidin–biotin interaction is leveraged. This compound is essential for life science researchers, diagnostic kit developers, and contract manufacturing organizations producing biotinylated probes, peptides, oligonucleotides, and glycans. As a key intermediate in the production of biotinylated conjugates, it enables reproducible, scalable, and GMP-compliant bioconjugation processes.

Application

• Site-specific biotinylation of serine-, threonine-, or carbohydrate-derived hydroxyl groups in peptides and glycoproteins
• Preparation of biotinylated solid-phase synthesis supports for oligonucleotide and peptide arrays
• Immobilization of hydroxyl-functionalized ligands onto streptavidin-coated biosensor surfaces (e.g., SPR, QCM, ELISA plates)
• Synthesis of biotin-ethanol spacer arms for antibody–biotin conjugates in IVD assay development
• Chemical modification of polysaccharides (e.g., dextran, hyaluronic acid) for targeted drug delivery systems
• Production of biotinylated standards and calibrators for mass spectrometry-based biomarker quantification
• Labeling of small-molecule metabolites bearing primary alcohol moieties for pull-down and interactome studies

Basic Information

Product Name	2-(Biotinylamido)Ethanol
CAS No.	95611-10-2
Molecular Formula	C12H22N4O4S
Molecular Weight	318.40 g/mol
Synonyms	Biotin-PEO-ethanol; Biotinamidoethanol; 2-(Biotinamido)ethanol; Biotinylaminoethanol; N-(2-Hydroxyethyl)biotinamide; Biotin-PEG1-ethanol; Biotin-ethanolamine; Biotinyl ethanolamine; Biotin-HEA; Biotin-hydroxyethylamine
EINECS	602-754-2

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (IR, HPLC retention time), purity (≥98.0% by HPLC), residual solvents (meets ICH Q3C guidelines), and bioburden (sterile filtration optional upon request). Testing follows ACS Reagent Grade and USP General Chapter <1058> Analytical Instrument Qualification principles where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (nitrogen or argon) to prevent oxidation. Due to its hygroscopic nature, minimize exposure to ambient humidity; use desiccated storage conditions and handle only in a dry nitrogen glovebox or under controlled low-humidity environment. Avoid repeated opening of the container.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥98.0%
Related Substances (HPLC)	Single impurity ≤0.5%; Total impurities ≤2.0%
Residue on Ignition	≤0.1%
Water (Karl Fischer)	≤1.0%
Heavy Metals	≤10 ppm
Specific Optical Rotation	[α]²⁵D = –15° to –20° (c = 1 in water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.