Description

Resorufin β-D-Galactopyranoside is a specialized fluorogenic substrate used for the detection and quantification of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where high sensitivity and specificity are required. It is primarily utilized by biotechnology, pharmaceutical, and diagnostic industries for enzyme-linked assays, cell-based reporter gene studies, and high-throughput screening.

Application

• Reporter Gene Assays: A key substrate for detecting β-galactosidase expression in molecular biology and genetic engineering.
• Enzyme Activity Measurement: Used in biochemical research to quantify β-galactosidase activity with high sensitivity via fluorometric detection.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify modulators of gene expression or enzyme function.
• Cell-Based Assays: Facilitates the study of gene regulation and promoter activity in live cells.
• Diagnostic Kits: Serves as a component in diagnostic test kits for bacterial detection (e.g., E. coli) and other enzymatic markers.
• Microbiology Research: Used as a chromogenic/fluorogenic agent in microbial identification and studies of lacZ gene expression.

Basic Information

Product Name	Resorufin β-D-Galactopyranoside
CAS No.	95079-19-9
Molecular Formula	C19H17NO9
Molecular Weight	403.34 g/mol
Synonyms	Resorufin β-D-Galactopyranoside; Resorufin β-D-Galactoside; β-Galactosidase Substrate Resorufin; 7-Hydroxy-3H-phenoxazin-3-one 1-β-D-galactopyranoside; 3H-Phenoxazin-3-one, 7-hydroxy-, 1-β-D-galactopyranoside; Resorufin Gal; RBG; Fluorogenic β-Gal Substrate
EINECS	Contact for details

Quality Control

Our Resorufin β-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/IR)	Conforms to standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorogenic Activity	Functional test passed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.