Description

Cyclophilin A CAS NO 95076-93-0 is a peptidyl-prolyl cis-trans isomerase (PPIase) enzyme that plays a critical role in protein folding and cellular signaling. Its function as a key immunophilin makes it an essential research tool and a target for therapeutic development. This high-purity protein is vital for researchers and manufacturers in pharmaceutical development, immunology research, and biochemistry, particularly in studies involving immunosuppression, viral replication, and protein interaction networks.

Application

• Drug Discovery & Pharmaceutical Research: Primary target for immunosuppressive drugs like Cyclosporin A; used in screening assays and mechanism-of-action studies.
• Biochemical & Enzymology Studies: Serves as a standard enzyme for investigating PPIase activity, protein folding kinetics, and chaperone functions.
• Immunology & Cell Signaling Research: Used to study its role in T-cell activation, inflammatory responses, and intracellular signaling pathways.
• Virology Research: Critical component in research on HIV-1 and other viruses that utilize Cyclophilin A for replication and capsid assembly.
• Protein-Protein Interaction Analysis: Employed in assays like SPR, ELISA, and pull-down experiments to map interaction partners and complexes.
• Diagnostic Reagent Development: Used as a purified antigen or component in developing diagnostic kits for autoimmune or inflammatory conditions.

Basic Information

Product Name	Cyclophilin A
CAS No.	95076-93-0
Molecular Formula	CnHnNnOnSn (Protein)
Molecular Weight	~18 kDa
Synonyms	Peptidyl-prolyl cis-trans isomerase A; PPIA; Rotamase A; CYPA; 18 kDa Immunophilin; Cyclosporin A-binding protein; Cyclophilin-18; T-Cell Cyclophilin; PPIase A; hCYPA; EC 5.2.1.8
EINECS	Contact for details

Quality Control

Our Cyclophilin A is produced under stringent conditions to ensure high biological activity and purity, suitable for sensitive research applications. Each batch is characterized by multiple analytical techniques to confirm identity, purity, and functional activity. A comprehensive Certificate of Analysis (COA) detailing specifications such as purity (by SDS-PAGE and HPLC), specific activity (PPIase assay), endotoxin level, and bioburden is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. For reconstituted solutions, aliquot and store at -70°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use in a low-humidity environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~18 kDa
Identification (Mass Spec)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Purity (SDS-PAGE)	≥ 98%
Specific Activity (PPIase)	≥ [Value] units/mg
Endotoxin	< 1.0 EU/μg
Bioburden	To meet USP/EP guidelines
Solubility	Soluble in aqueous buffers
Protein Content (BCA)	≥ 90% of stated mass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.