Description

Human Tnf-Alpha CAS NO 94948-61-5 is a highly purified, recombinant cytokine protein, specifically tumor necrosis factor-alpha, essential for advanced biomedical research and therapeutic development. This critical signaling molecule plays a pivotal role in regulating immune responses, inflammation, and cellular apoptosis. It is indispensable for researchers and developers in the pharmaceutical, biotechnology, and diagnostic sectors, enabling groundbreaking work in immunology, oncology, and autoimmune disease studies.

Application

• Biomedical Research: Used as a key reagent in cell culture studies to investigate inflammatory pathways, apoptosis, and immune cell activation.
• Drug Discovery & Development: Serves as a target or stimulant in high-throughput screening assays for developing anti-inflammatory and anti-cancer therapeutics.
• Diagnostic Kit Manufacturing: Employed as a critical standard or calibrator in ELISA and other immunoassays for quantifying TNF-α levels in clinical samples.
• Cell-Based Therapy Research: Applied in studies exploring CAR-T cell and other immunotherapies to understand and modulate T-cell function.
• Academic & Institutional Research: Fundamental tool for studying the molecular mechanisms of sepsis, rheumatoid arthritis, Crohn's disease, and other TNF-α mediated conditions.
• Biologics Production: Used in quality control and potency testing during the manufacturing of monoclonal antibody drugs that target TNF-α.

Basic Information

Product Name	Human Tnf-Alpha
CAS No.	94948-61-5
Molecular Formula	C642H998N174O196S6
Molecular Weight	Approx. 17.5 kDa (monomer)
Synonyms	Tumor Necrosis Factor Alpha; TNF-α; TNF-a; Cachectin; TNFSF2; TNF Superfamily Member 2; Recombinant Human TNF-Alpha; rhTNF-α; TNF, Human; Tumor Necrosis Factor Ligand Superfamily Member 2
EINECS	Contact for details

Quality Control

Our Human TNF-Alpha is produced under stringent conditions to ensure the highest levels of purity, biological activity, and consistency. Each batch is rigorously tested for identity, purity (≥95% by SDS-PAGE and HPLC), endotoxin levels (<0.1 EU/µg), and specific biological activity. Certificates of Analysis (COA) detailing all quality parameters are provided to guarantee compliance with research-grade and cGMP guidelines for non-clinical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C to -80°C. Reconstituted solutions should be aliquoted and stored at -80°C to avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to ~17.5 kDa
Purity (HPLC)	≥ 95%
Biological Activity	Measured in a standardized cell cytotoxicity assay (e.g., L929 mouse fibroblast assay)
Endotoxin Level (LAL)	< 0.1 EU/µg
Protein Content (UV Absorbance)	Determined by A280 measurement
Solubility	Soluble in sterile PBS or cell culture medium
Sterility (Membrane Filtration)	Sterile-filtered, 0.2 µm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.