Description

Sir3 Protein CAS NO 94895-23-5 is a high-purity, biologically active protein derived from Saccharomyces cerevisiae, playing a crucial role in chromatin structure and gene silencing. This product is essential for researchers and developers in the life sciences sector requiring consistent, high-quality protein for mechanistic studies and assay development. It is primarily utilized in biochemical research, pharmaceutical R&D for epigenetic target validation, and as a critical component in enzymatic and binding assays.

Application

• Biochemical Research: Used as a key reagent in studies of chromatin remodeling, heterochromatin formation, and gene silencing mechanisms.
• Pharmaceutical R&D: Serves as a target protein for screening and validating novel epigenetic modulators and therapeutic candidates.
• Enzymatic Assays: Employed in activity assays to study histone deacetylase (HDAC) complex functions and kinetics.
• Protein-Protein Interaction Studies: Utilized in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interactomes.
• Diagnostic Development: Acts as a component in developing diagnostic kits for research into diseases linked to epigenetic dysregulation.
• Academic Research: Fundamental tool for university and institutional labs exploring yeast genetics and epigenetics.

Basic Information

Product Name	Sir3 Protein
CAS No.	94895-23-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Silent Information Regulator Protein 3; SIR3; Yeast Sir3 Protein; Chromatin Silencing Protein Sir3; Regulatory Protein SIR3; S. cerevisiae Sir3; Sir3p; NAD-dependent protein deacetylase sir3; EC 3.5.1.98
EINECS	Contact for details

Quality Control

Our Sir3 Protein is manufactured under stringent quality management systems. Each batch undergoes rigorous analytical testing, including purity assessment by SDS-PAGE and HPLC, activity verification via functional assays, and confirmation of identity. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single major band at appropriate molecular weight
Purity (HPLC)	≥ 90%
Protein Concentration	As per COA (typically 0.1-1.0 mg/mL)
Biological Activity	Confirmed by functional assay (e.g., binding or enzymatic activity)
Endotoxin Level	< 1.0 EU/μg (for applicable grades)
Buffer Composition	Specified on COA (e.g., Tris, NaCl, Glycerol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.