Description

6-Hydroxy Warfarin-D5 is a high-purity, stable isotope-labeled analog of the primary metabolite of the anticoagulant drug warfarin. This compound is an essential reference standard and internal standard for precise quantitative analysis in pharmaceutical research and bioanalytical studies. It is primarily used by analytical chemists and researchers in pharmaceutical development, clinical laboratories, and contract research organizations (CROs) for method development, validation, and pharmacokinetic studies.

Application

• LC-MS/MS Internal Standard: Used as a deuterated internal standard for the accurate quantification of 6-hydroxywarfarin and related metabolites in biological matrices (e.g., plasma, urine) via mass spectrometry, correcting for matrix effects and instrument variability.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Critical for tracing the metabolic pathways of warfarin, enabling studies on inter-individual variability in CYP2C9 enzyme activity and understanding drug-drug interactions.
• Bioanalytical Method Development & Validation: Serves as a key component in developing robust, sensitive, and specific HPLC-MS/MS or GC-MS methods for clinical and preclinical sample analysis.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Facilitates precise measurement of warfarin metabolite levels in patient samples, aiding in personalized anticoagulant therapy and dose optimization.
• Pharmaceutical Quality Control: Employed as a certified reference material (CRM) to ensure the accuracy and traceability of analytical results in pharmaceutical manufacturing and impurity profiling.
• Forensic and Toxicological Analysis: Used in specialized laboratories for the definitive identification and quantification of warfarin and its metabolites in forensic investigations.

Basic Information

Item	Detail
Product Name	6-Hydroxy Warfarin-D5
CAS No.	94820-64-1
Molecular Formula	C19H11D5O6
Molecular Weight	313.33 g/mol (approx.)
Synonyms	6-Hydroxywarfarin-d5; 4-Hydroxy-3-(3-oxo-1-phenylbutyl)-2H-1-benzopyran-2-one-d5 (6-hydroxy); Warfarin 6-hydroxy metabolite-d5; 6-OH Warfarin-D5; (RS)-6-Hydroxywarfarin-d5; 3-(α-Acetonylbenzyl)-4-hydroxycoumarin-6-hydroxy-d5; 6-Hydroxy-3-(α-phenyl-β-acetylethyl)-4-hydroxycoumarin-d5
EINECS	Contact for details

Quality Control

Every batch of 6-Hydroxy Warfarin-D5 is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to support its use as a high-grade reference standard. Certificates of Analysis (COA) are supplied with each shipment, detailing critical parameters such as isotopic purity, chemical purity, and identity confirmation via orthogonal methods. Our quality commitment ensures consistency and reliability for your most demanding analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. For long-term storage, consider storing at -20°C under inert atmosphere to maintain optimal stability and isotopic integrity. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0% (chemical purity)
Isotopic Purity (D-content)	≥ 98.0 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.