Description

α-Hemolysin is a potent, pore-forming exotoxin primarily produced by *Staphylococcus aureus*. This compound is of significant value in biomedical research for studying bacterial pathogenesis, host cell lysis mechanisms, and membrane protein interactions. It is an essential reagent for researchers and developers in the fields of microbiology, immunology, and novel therapeutic discovery, particularly for investigating cytolytic activity and developing toxin-based delivery systems.

Application

• Biomedical Research: Fundamental studies on bacterial virulence, pore-forming toxin mechanisms, and host-pathogen interactions.
• Cell Biology & Cytotoxicity Assays: Used as a controlled lytic agent to permeabilize cell membranes in research applications.
• Drug Delivery Platform Development: Investigation of engineered toxin pores for biosensing or targeted macromolecule delivery.
• Vaccine & Antitoxin Research: Serves as a key antigen in the development of vaccines and therapeutic antibodies against S. aureus infections.
• Membrane Biophysics: A model protein for studying ion channel formation, single-molecule sensing, and lipid bilayer dynamics.
• Diagnostic Reagent Component: Potential use in diagnostic kits for detecting specific bacterial strains or their pathogenic factors.

Basic Information

Product Name	α-Hemolysin
CAS No.	94716-94-6
Molecular Formula	Contact for details
Molecular Weight	~33 kDa (Monomer)
Synonyms	α-Toxin; Staphylococcal α-hemolysin; Hla; α-hemolysin (S. aureus); α-lysin; α-haemolysin; S. aureus α-toxin; α-hemolytic toxin
EINECS	Contact for details

Quality Control

Our α-Hemolysin is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and biological activity. Each lot is subjected to rigorous purity analysis and functional activity testing. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and specific activity is provided with every shipment to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at ≤ -70°C to prevent repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure containers are sealed under anhydrous conditions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Corresponds to standard
Purity (HPLC)	≥ 95%
Protein Content	≥ 90%
Specific Hemolytic Activity	Defined per batch
Endotoxin Level	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.